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MAK120

Sigma-Aldrich

Urease Activity Assay Kit

sufficient for 100 colorimetric tests

Synonyme(s) :

Urea Amidohydrolase Activity Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric tests

Application(s)

environmental

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

gastrointestinal diseases

Température de stockage

2-8°C

Catégories apparentées

Description générale

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in bacteria, yeast, and certain higher plant species. Urease activity is found in environmental and fecal samples.

Application

Urease Activity Assay Kit has been used to measure urease activity.

Caractéristiques et avantages

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Adéquation

Suitable for the detection of Urease activity in biological and environmental samples.

Principe

In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.

Pictogrammes

Health hazardCorrosionEnvironment
GHS08,GHS05,GHS09

Mention d'avertissement

Danger

Mentions de danger

H290,H314,H341,H410

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Risques supp

EUH031

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Laura Zanin et al.
Frontiers in plant science, 7, 845-845 (2016-07-23)
To limit nitrogen (N) losses from the soil, it has been suggested to provide urea to crops in conjunction with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT). However, recent studies reported that NBPT affects urea uptake and urease activity in
James M Wiebler et al.
Proceedings. Biological sciences, 285(1878) (2018-05-04)
Gut bacteria that produce urease, the enzyme hydrolysing urea, contribute to nitrogen balance in diverse vertebrates, although the presence of this system of urea-nitrogen recycling in Amphibia is as yet unknown. Our studies of the wood frog (Rana sylvatica), a
Bhawna Uprety et al.
Pharmaceutics, 14(1) (2022-01-22)
Tumour cells maintain a local hypoxic and acidic microenvironment which plays a crucial role in cancer progression and drug resistance. Urease is a metallohydrolases that catalyses the hydrolysis of urea into ammonia and carbon dioxide, causing an abrupt increase of
Satya P G
Hydroxamic Acids: A Unique Family of Chemicals with Multiple Biological Activities (2013)
Isolation, partial purification, characterization and inhibition of urease (EC 3.5. 1.5) enzyme from the Cajanus cajan seeds.
Sujoy B and Aggarwal A
Asian Journal of Bio Science, 7(2), 203-209 (2012)
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