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T7782

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–TRITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
MFCD00162624
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

200

Conjugué

TRITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Conditions de stockage

protect from light

Technique(s)

direct immunofluorescence: 1:64
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Catégories apparentées

Description générale

Immunoglobulin G (IgG)antibody is found in four subclasses IgG1, IgG2, IgG3 and IgG4. It has a monomeric structure. IgG is secreted by B cells and is found in blood and extracellular fluids.. Anti-Mouse IgG (Fab specific) is conjugated to tetramethylrhodamine isothiocyanate (TRITC) and then purified by gel filtration to remove free TRITC.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Conjugation of TRITC to Mouse IgG (Fab specific) antibody enables the use in immunofluorescent techniques.

Actions biochimiques/physiologiques

IgG antibody subtype provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) antibody participates in complement fixation and opsonization.

Autres remarques

Antibody adsorbed with bovine, equine and human serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Notes préparatoires

Adsorbed to reduce background staining with bovine, horse or human samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Sh Hejazi et al.
Iranian journal of parasitology, 7(3), 16-23 (2012-10-31)
Cutaneous leishmaniasis is a neglected parasitic disease, which imposes massive human distress and financial costs to the endemic countries. Better understanding of host immune response to the parasite leads to helpful strategies for disease control. Interleukin (IL)-10 and transforming growth
Sh Hejazi et al.
Iranian journal of parasitology, 7(2), 53-60 (2012-10-31)
Cutaneous leishmaniasis is a neglected parasitic disease, which imposes massive human distress and financial costs to the endemic countries. Better understanding of host immune response to the parasite leads to helpful strategies for disease control. Interleukin (IL)-10 and transforming growth
Yi-Liang Miao et al.
Environmental and molecular mutagenesis, 50(8), 666-671 (2009-07-14)
Centrosomes are critically important for maintaining meiotic spindle integrity in the meiosis II (MII) stage where oocytes are arrested in most mammalian species before fertilization takes place. In women of advanced ages or during in vitro fertilization (IVF) procedures, aneuploidy
Hua Jiang et al.
Molecular oncology, 8(2), 297-310 (2014-01-07)
Reprogramming of NK cells with a chimeric antigen receptor (CAR) proved an effective strategy to increase NK cell reactivity and recognition specificity toward tumor cells. To enhance the cytotoxicity of NK cells against CD138-positive multiple myeloma (MM) cells, we generated
Shervin G Hoseini et al.
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 17(6), 513-518 (2013-04-30)
Cutaneous leishmaniasis (CL) is a public health problem in several endemic countries. Recent studies on mouse model and also a few clinical experiments showed that the type of immune response generated at the site of infection and especially balance between
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