T1952

Trichostatin A, Ready Made Solution

5 mM in DMSO, from Streptomyces sp.

• Synonyme(s) : TSA, Trichostatin A, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
• Formule empirique (notation de Hill): C₁₇H₂₂N₂O₃
• Numéro CAS: 58880-19-6
• Poids moléculaire : 302.37
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: Streptomyces sp.
• Niveau de qualité: 200
• Pureté: ≥98% (HPLC)
• Forme: DMSO solution
• Concentration: 5 mM in DMSO
• Technique(s): cell culture | mammalian: suitable
• Spectre d'activité de l'antibiotique: fungi; neoplastics
• Mode d’action: enzyme | inhibits
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• InChI: 1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
• Clé InChI: RTKIYFITIVXBLE-WKWSCTOISA-N

Description

Description générale

Trichostatin A is a compound of primary hydroxamic acid.

Application

Trichostatin A, ready-made solution has been used:

• as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing
• to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)
• to treat cells for the HDAC inhibition experiments

Actions biochimiques/physiologiques

Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)