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Key Documents

SRP6539

Sigma-Aldrich

NQO1 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonyme(s) :

Azoreductase, DT-Diaphorase (DTD), Dioxin-inducible 1, Disphorase-4 (DIA4), Menadione Reductase, NAD(P)H Dehydrogenase (Quinine) 1 (NQO1), NAD(P)H Quinone Oxireductase, Phylloquinone Reductase, Quinone Oxidoreductase 1, Quinone Reductase 1 (QR1)

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥90% (SDS-PAGE)

Forme

liquid

Poids mol.

~30 kDa (Human NQO1 aa 1-274 is fused at the C-terminus to a His-tag.)

Conditionnement

pkg of 10 and 50 μg

Impuretés

<0.1 EU/μg endotoxin (LAL test)

Numéro d'accès UniProt

P15559

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... NQO1(1728)

Description générale

Research area: Cell signaling. NQO1 (NAD(P)H quinone dehydrogenase 1) is an obligate two-electron reductase. It is a cytoplasmic, homodimeric protein, with one FAD molecule per monomer. This gene is localized to human chromosome 16q23. It shows a wide range of tissue expression.

Actions biochimiques/physiologiques

NQO1 (NAD(P)H quinone dehydrogenase 1) uses NADH or NADPH as a reducing cofactor, and is characterized by its inhibition by dicoumarol. It is thought to confer protection against natural and exogenous quinones. It is involved in the conversion of benzene derived quinones to less toxic hydroquinones, and participates in benzene-associated hematotoxicity. It is involved in the reduction and eventual activation of few chemotherapeutic drugs and of environmental carcinogens, such as heterocyclic amines, nitroaromatic compounds, and possibly cigarette smoke particulate. C609T null variant of this gene is linked with breast cancer susceptibility in north Indian patients. NQO1 is up-regulated in serous ovarian carcinoma, and this expression is linked with poor prognosis; hence, this gene has potential as biomarker for the same.

Forme physique

0.2 μm-filtered solution in 50 mM Tris-Cl, pH 8.0, containing 1 mM DTT.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), a multifunctional antioxidant enzyme and exceptionally versatile cytoprotector.
Dinkova-Kostova AT and Talalay P.
Archives of Biochemistry and Biophysics, 501(1), 116-123 (2010)
High expression of NQO1 is associated with poor prognosis in serous ovarian carcinoma.
Cui X, et al.
BMC Cancer, 15:244 (2015)
Prevalence of the inactivating 609C-->T polymorphism in the NAD(P)H:quinone oxidoreductase (NQO1) gene in patients with primary and therapy-related myeloid leukemia.
Larson RA, et al.
Blood, 94(2), 803-807 (1999)
The C609T (Pro187Ser) Null Polymorphism of the NQO1 Gene Contributes Significantly to Breast Cancer Susceptibility in North Indian Populations: a Case Control Study.
Yadav P, et al.
Asian Pacific Journal of Cancer Prevention, 17(3), 1215-1219 (2016)
Xiang Li et al.
Bioorganic & medicinal chemistry, 24(5), 1006-1013 (2016-01-25)
A new series of ortho-naphthoquinone analogs of β-lapachone were designed, synthesized and evaluated. The biological results indicated that most of our compounds were efficient substrates for NQO1. The new scaffold with water-soluble side chain resulted in greater solubility under acidic
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