N9284
Nitroreductase from Escherichia coli
≥90% (SDS-PAGE), recombinant, expressed in E. coli
Synonyme(s) :
NTRA
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Produit recombinant
expressed in E. coli
Niveau de qualité
Pureté
≥90% (SDS-PAGE)
Forme
lyophilized powder
Activité spécifique
≥100 units/mL
Poids mol.
monomer 24000
Caractéristiques du produit alternatif plus écologique
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Numéro d'accès UniProt
Autre catégorie plus écologique
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
Escherichia coli K12 ... nfsB(945483)
Description générale
Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.
Application
Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.
Actions biochimiques/physiologiques
Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Propriétés physiques
Lyophilized powder containing PBS. Does not contain BSA as excipient
Définition de l'unité
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
Notes préparatoires
Produced using animal component-free materials.
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Les clients ont également consulté
Chih-Chen Chen et al.
Food chemistry, 135(4), 2708-2713 (2012-09-18)
Nitroreductases (Nrs) play important roles in redox system via NADPH or NADH as a reductant. A TcNr cDNA encoding a putative Nr was cloned from Taiwanofungus camphorata. A 3-D structural model of the TcNr has been created based on the
Dual enzyme-responsive ?turn-on? fluorescence sensing systems based on in situ formation of 7-hydroxy-2-iminocoumarin scaffolds
Debieu, S and Romieu A
Organic & Biomolecular Chemistry, 13(41), 10348-10361 (2015)
Generation of Escherichia coli nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954
Grove JI, et al.
Cancer research, 63(17), 5532-5537 (2003)
Mckayla Stevens et al.
Bioorganic & medicinal chemistry, 28(22), 115710-115710 (2020-10-03)
In two previous studies, we identified compound 1 as a moderate GroEL/ES inhibitor with weak to moderate antibacterial activity against Gram-positive and Gram-negative bacteria including Bacillus subtilis, methicillin-resistant Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, and SM101 Escherichia coli (which has
Bharat Bhushan et al.
Biochemical and biophysical research communications, 322(1), 271-276 (2004-08-18)
Previously, we reported that a salicylate 1-monooxygenase from Pseudomonas sp. ATCC 29352 biotransformed CL-20 (2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaaza-isowurtzitane) (C(6)H(6)N(12)O(12)) and produced a key metabolite with mol. wt. 346 Da corresponding to an empirical formula of C(6)H(6)N(10)O(8) which spontaneously decomposed in aqueous medium to
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