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Key Documents

SRP0300

Sigma-Aldrich

TGF-β1 Latent human

recombinant, expressed in FreeStyle™ 293-F cells, ≥98% (SDS-PAGE)

Synonyme(s) :

LAP, TGFb1, TGFbeta1, latency-associated peptide, transforming growth factor beta-1

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in FreeStyle™ 293-F cells

Pureté

≥98% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

25 kDa

Conditionnement

pkg of 5 μg

Fabricant/nom de marque

Invitrogen

Conditions de stockage

avoid repeated freeze/thaw cycles

Numéro d'accès NCBI

NM_000660

Numéro d'accès UniProt

Q14766

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... LTBP1(4052)

Description générale

Human TGFβ1 (GenBank Accession No. NM_000660), amino acids 30-390 (end) with Cys33Ser mutation and N-terminal His-tag, disulfide-linked homodimers of TGFβ1 LAP and mature TGFβ1, MW= ~75kDa and 25kDa, under non-reducing conditions.
LTBP1 (latent transforming growth factor β binding protein 1) belongs to the LTBP family of proteins. The protein has 18 epidermal growth factor (EGF)-like repeats and four LTBP-like repeats. The gene is mapped to human chromosome 2p22.3.

Application

Useful for cell culture and for the study of signaling and apoptotic pathways.

Actions biochimiques/physiologiques

LTBP1 (latent transforming growth factor β binding protein 1) is an extracellular matrix protein which also controls TGF-β (transforming growth factor β) bioavailability. LTBP1 is mainly responsible for the folding and secretion of TGF-β, thereby adding it to the extracellular matrix. It is a target of HTRA1 (high temperature requirement protein A1), a serine protease involved in cellular signaling.

Forme physique

Lyophilized from a 0.2 μM filtered, aqueous solution.

Reconstitution

Reconstitute in sterile PBS with 0.1% BSA. Stability: >6 months at –70°C (as a solid). Store reconstituted protein in aliquots at –80°C for up to 3 months. Avoid freeze/thaw cycles.

Informations légales

FreeStyle is a trademark of Invitrogen Corp.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Cerebral small vessel disease-related protease HtrA1 processes latent TGF-? binding protein 1 and facilitates TGF-? signaling.
Beaufort N, et al.
Proceedings of the National Academy of Sciences of the USA, 111, 16496-16501 (2014)
Association of the small latent transforming growth factor-beta with an eight cysteine repeat of its binding protein LTBP-1.
Saharinen J, et al.
The Embo Journal, 15, 245-253 (1996)
A suite of novel human spermatozoal RNAs.
Ostermeier GC, et al.
Journal of Andrology, 26, 70-74 (2005)
J S Munger et al.
Kidney international, 51(5), 1376-1382 (1997-05-01)
Transforming growth factor-beta are cytokines with a wide range of biological effects. They play a pathologic role in inflammatory and fibrosing diseases such as nephrosclerosis. TGF-beta s are secreted in a latent form due to noncovalent association with latency associated
L Bourdrel et al.
Protein expression and purification, 4(2), 130-140 (1993-04-01)
A partial cDNA clone encoding the gene for human transforming growth factor-beta 1 (TGF-beta 1) was isolated from a human bladder carcinoma cell line (5637) cDNA library. Following restriction enzyme processing and ligation of synthetic oligonucleotide linkers, the gene was
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