SMB00610

Lipopolysaccharide from Porphyromonas gingivalis

purified by phenol extraction

• Synonyme(s) : LPS
• Code UNSPSC : 12352211
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: Porphyromonas gingivalis
• Niveau de qualité: 200
• Forme: powder
• Produit purifié par: phenol extraction
• Impuretés: ≤3% Protein (Lowry)
• Couleur: white to faint yellow
• Solubilité: triethylene glycol dimethyl ether: 0.90-1.10 mg/mL to hazy, colorless to light yellow
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria like Porphyromonas gingivalis. It differs from LPS from Escherichia coli in its structure and various functional activities.

Application

Lipopolysaccharide has been used:

• as a treatment to stimulate acute lung injury in human bronchial epithelial (HBE) cells
• as an agonist for toll-like receptor 4 (TLR4) and to stimulate intestinal normal fibroblasts (NFs) to study osteopontin (OPN) expression in myofibroblasts
• to study its effects on pro-inflammatory and pro-coagulant genes expression in endothelial cells

Actions biochimiques/physiologiques

LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular patterns (PAMPs).

Additionally, it was demonstrated that the mechanisms by which LPS from E. coli and P. gingivalis modulate cluster of differentiation 14 (CD14), toll-like receptor 2 (TLR2), and toll-like receptor 4 (TLR4) surface expression, primary and secondary cytokine responses are different.

Porphyromonas gingivalis is a Gram-negative bacterium that is known to be involved in adult periodontitis. Periodontitis is a chronic inflammatory disease characterized by the recession of the supportive tissue surrounding teeth. Studies have shown that the LPS from P. gingivalis plays an important role in this disease.
A recent study demonstrated that LPS from P. gingivalis stimulates insulin secretion by the pancreatic β cell line, MIN6. In the presence of 5 mM glucose and 50-500 ng/mL LPS from P. gingivalis, a significant induction of insulin secretion was observed.

Notes préparatoires

LPS from P. gingivalis is soluble in water and cell culture medium DMEM (1-5 mg/mL), yielding a clear solution.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable