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P5412

Sigma-Aldrich

o-Phenylenediamine

peroxidase substrate, chromogenic, tablet

Synonyme(s) :

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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About This Item

Formule empirique (notation de Hill):
C6H8N2
Numéro CAS:
95-54-5
Poids moléculaire :
108.14
Beilstein:
606074
Numéro CE :
202-430-6
Numéro MDL:
MFCD00007721
Code UNSPSC :
12352204
ID de substance PubChem :
24898638
Nomenclature NACRES :
NA.83
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

o-Phenylenediamine, tablet, 20 mg substrate per tablet

Densité de vapeur

3.7 (vs air)

Niveau de qualité

200

Pression de vapeur

0.01 mmHg ( 25 °C)

Forme

tablet

pb

256-258 °C

Pf

100-102 °C

Fluorescence

λex 337 nm; λem 417 nm (after derivatization)

Température de stockage

2-8°C

Chaîne SMILES 

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

Clé InChI

GEYOCULIXLDCMW-UHFFFAOYSA-N

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Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
o-Phenylenediamine has been used to detect transforming growth factor (TGF-β)[1] and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA).[2]

Actions biochimiques/physiologiques

o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation.[3]

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Pictogrammes

Skull and crossbonesHealth hazardEnvironment
GHS06,GHS08,GHS09

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

276.8 °F - closed cup

Point d'éclair (°C)

136 °C - closed cup

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Certificats d'analyse (COA)

Lot/Batch Number
0000368791
0000368793
0000258062
0000258062
0000368793

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Application of o-phenylenediamine as a fluorogenic substrate in peroxidase-mediated enzyme-linked immunosorbent assay
Mekler VM and Bystryak SM
Analytica Chimica Acta, 264(2), 359-363 (1992)
Interleukin-13 Regulates Secretion of the Tumor Growth Factor-beta Superfamily Cytokine Activin A in Allergic Airway Inflammation
Hardy CL, et al.
American Journal of Respiratory Cell and Molecular Biology, 42(6), 667-675 (2010)
Receptor-mediated abeta amyloid antibody targeting to Alzheimer?s disease mouse brain
Zhou QH, et al.
Molecular Pharmaceutics, 8(1), 280-285 (2010)
Jonas R Knudsen et al.
International journal of molecular sciences, 21(7) (2020-04-02)
The small molecule kinase inhibitor SBI-0206965 was originally described as a specific inhibitor of ULK1/2. More recently, it was reported to effectively inhibit AMPK and several studies now report its use as an AMPK inhibitor. Currently, we investigated the specificity
Fu Ci Guo et al.
Diseases of aquatic organisms, 61(1-2), 175-178 (2004-12-09)
The course of Spironucleus barkhanus (Diplomonadida: Hexamitidae) infection in Atlantic salmon Salmo salar L. (Salmonidae) has 2 distinct phases, a blood phase and a tissue phase. To detect and quantify an infection, 3 parasitological techniques, namely Wet Mount Examination (WME)
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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