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P9029

Sigma-Aldrich

o-Phenylenediamine

peroxidase substrate, chromogenic, ≥98.0%, powder

Synonyme(s) :

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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About This Item

Formule empirique (notation de Hill):
C6H8N2
Numéro CAS:
95-54-5
Poids moléculaire :
108.14
Numéro Beilstein :
606074
Numéro CE :
202-430-6
Numéro MDL:
MFCD00007721
Code UNSPSC :
12352204
ID de substance PubChem :
24899004
Nomenclature NACRES :
NA.83

product name

o-Phenylenediamine, Peroxidase substrate, ≥98.0%, powder

Densité de vapeur

3.7 (vs air)

Niveau de qualité

200

Pression de vapeur

0.01 mmHg ( 25 °C)

Pureté

≥98.0%

Forme

powder

Couleur

white to off-white

Point d'ébullition

256-258 °C

Pf

98-102 °C

Solubilité

ethanol: 50 mg/mL, clear, colorless to faintly yellow

Fluorescence

λex 337 nm; λem 417 nm (Pyruvic acid derivative)

Température de stockage

2-8°C

Chaîne SMILES 

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

Clé InChI

GEYOCULIXLDCMW-UHFFFAOYSA-N

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Description générale

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.

Attention

May darken in storage.

Pictogrammes

Skull and crossbonesHealth hazardEnvironment
GHS06,GHS08,GHS09

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

276.8 °F - closed cup

Point d'éclair (°C)

136 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Kelsey E Noll et al.
Cell reports, 31(4), 107587-107587 (2020-04-30)
Host genetic factors play a fundamental role in regulating humoral immunity to viral infection, including influenza A virus (IAV). Here, we utilize the Collaborative Cross (CC), a mouse genetic reference population, to study genetic regulation of variation in antibody response
Bothrops asper envenoming in cattle: Clinical features and management using equine-derived whole IgG antivenom
C.Rodriguez, et al.
The Veterinary Journal, 207(, pages=), 160-163 (2016)
Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom
Navarro D, et al.
Toxicon, 120(3), 1-8 (2016)
Mehul S Suthar et al.
Cell reports. Medicine, 1(3), 100040-100040 (2020-08-25)
SARS-CoV-2, the virus responsible for COVID-19, is causing a devastating worldwide pandemic, and there is a pressing need to understand the development, specificity, and neutralizing potency of humoral immune responses during acute infection. We report a cross-sectional study of antibody
Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors
Jacobson A, et al.
International Journal of Cancer. Journal International Du Cancer, 102(3), 212-219 (2002)
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