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P1620

Sigma-Aldrich

Anti-PRMT1 antibody, Mouse monoclonal

clone PRMT1-171, purified from hybridoma cell culture

Synonyme(s) :

Anti-Protein-Arginine Methyl Transferase 1

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About This Item

Numéro MDL:
MFCD04975120
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

PRMT1-171, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~42 kDa

Espèces réactives

mouse, chicken, bovine, human, canine, hamster, monkey, rat

Concentration

~2 mg/mL

Technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 2 μg/mL using whole cell extract of 293T cells

Isotype

IgG1

Numéro d'accès UniProt

Q99873

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PRMT1(3276)

Description générale

Protein arginine methyltransferase 1 (PRMT1) gene is localized on human chromosome 19q13.33. It is part of the type I PRMT family.

Spécificité

Does not cross-react with PRMT2, 3, 4, and 5.

Immunogène

full length PRMT1. Does not cross-react with PRMT2, 3, 4, and 5.

Application

Anti-PRMT1 antibody, Mouse monoclonal has been used for Immunofluorescence and Western Blotting.

Actions biochimiques/physiologiques

Protein arginine methyltransferase 1 (PRMT1) is crucial for the formation of asymmetric dimethylarginine. It can methylate Twist-1, a transcription factor. The protein also methylates histones and thus modulates transcription and protein activity. PRMT1 is involved in epithelial-mesenchymal transition and may have a role in cancer.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 C for up to one month. For prolonged storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

PRMT1-mediated arginine methylation controls ATXN2L localization.
Kaehler C, et al.
Experimental Cell Research, 334(1), 114-125 (2015)
Common fusion transcripts identified in colorectal cancer cell lines by high-throughput RNA sequencing.
Nome T, et al.
Translational Oncology, 6(5), 546-IN5-546-IN5 (2013)
PRMT1 Is a Novel Regulator of Epithelial-Mesenchymal-Transition in Non-small Cell Lung Cancer.
Avasarala S, et al.
The Journal of Biological Chemistry, 290(21), 13479-13489 (2015)
PRMT1 promotes glucose toxicity-induced β cell dysfunction by regulating the nucleo-cytoplasmic trafficking of PDX-1 in a FOXO1-dependent manner in INS-1 cells.
Lv L, et al.
Endocrine, 49(3), 669-682 (2015)
Li Liu et al.
Scientific reports, 6, 21810-21810 (2016-02-26)
Protein arginine methyltransferase 1 (PRMT1) catalyzes methylation of histones and other cellular proteins, and thus regulates gene transcription and protein activity. In antigen-induced pulmonary inflammation (AIPI) PRMT1 was up-regulated in the epithelium, while in chronic AIPI, increased PRMT1 shifted to
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