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P0089

Sigma-Aldrich

Anti-Prox1 antibody , Mouse monoclonal

clone 4G10, purified from hybridoma cell culture

Synonyme(s) :

Anti-Homeobox prospero-like protein Prox1, Anti-Prospero homeobox 1

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About This Item

Numéro MDL:
MFCD09039204
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

4G10, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~84 kDa

Espèces réactives

chicken, rat, lizard, human

Concentration

~1.5 mg/mL

Technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells
western blot: suitable

Isotype

IgG2b

Numéro d'accès UniProt

Q92786

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PROX1(5629)
rat ... Prox1(305066)

Description générale

Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero.

Immunogène

recombinant fusion protein corresponding to amino acids 547-737 of human Prox1.

Application

Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques.

Actions biochimiques/physiologiques

Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Loss of Function of the Candidate Tumor Suppressor prox1 by RNA Mutation in Human Cancer Cells
Takahashi M, et al.
Neoplasia, 8(12), 1003-1010 (2006)
Epigenetic silencing of the candidate tumor suppressor gene PROX1 in sporadic breast cancer
Versmold B, et al.
International Journal of Cancer. Journal International Du Cancer, 121(3), 547-554 (2007)
Molecular mechanisms of lymphatic vascular development
Makinen T, et al.
Cellular and Molecular Life Sciences, 64(15), 1915-1929 (2007)
Xiaoren Chen et al.
Hybridoma (2005), 25(1), 27-33 (2006-02-16)
Prox1 is a divergent homeodomain protein important for the development of the lens, retina, liver, pancreas, and lymphatic vasculature. Prox1 expression is highly upregulated in transformed hepatocytes and has been used as a marker to distinguish lymphatic from blood vasculature.
Guadalupe Álvarez-Hernán et al.
Cells, 12(7) (2023-04-14)
The quail (Coturnix coturnix, Linnaeus 1758), a notable model used in developmental biology, is a precocial bird species in which the processes of retinal cell differentiation and retinal histogenesis have been poorly studied. The purpose of the present research is
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