O8389

Anti-Oct 3/4 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-OTF3/OTF4, Anti-Octamer-binding transcription factor 3/4, Anti-POU class 5 homeobox 1, Anti-POU5F1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Espèces réactives: mouse, human, rat
• Concentration: ~1 mg/mL
• Technique(s): immunohistochemistry: 1:100-1:250; western blot: 1:500-1:1,000
• Numéro d'accès UniProt: Q01860
• Application(s): research pathology
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... POU5F1(5460) ; mouse ... Pou5f1(18999) ; rat ... Pou5f1(294562)

Description

Description générale

POU domain class 5 transcription factor 1 (POU5F1) is also termed as OCT4. It is a transcription factor. OCT4 codes for three isoforms, such as OCT4A, OCT4B and OCT4B1. These isoforms are produced by alternative splicing. The OCT4 gene consists of 7 exons. This gene is located on human chromosome 6. OCT4A and OCT4B are located in the nucleus and cytoplasm respectively.

Immunogène

synthetic peptide corresponding to an internal sequence of human Oct 3/4. This sequence is 84% identical in mouse and 94% identical in rat.

Application

Anti-Oct 3/4 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence staining.

Actions biochimiques/physiologiques

Oct 3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct 3/4 stimulates loss of pluripotency and dedifferentiation to trophectoderm. POU domain class 5 transcription factor 1 (POU5F1) serves as a master regulator of pluripotency in embryonic stem cells. It is one of the reprogramming factors, that is highly essential for generating induced pluripotent stem cells.

Forme physique

solution in phosphate buffered saline, containing 0.02% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)