N6288
Anti-NucleolinAtto 488
1.5-3.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Anti-NCL, PROTEIN C23
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
Atto 488 conjugate
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Espèces réactives
human, mouse, rat
Conditions de stockage
protect from light
Concentration
1.5-3.0 mg/mL
Technique(s)
direct immunofluorescence: 0.5-1.0 μg/mL using human HeLa, rat NRK and mouse 3T3 cells
Fluorescence
λex 500 nm; λem 522 nm in PBS
λex 500 nm; λem 522 nm
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... NCL(4691)
mouse ... Ncl(17975)
rat ... Ncl(25135)
Description générale
Nucleolin (NCL) is an anti-apoptotic phosphoprotein. It is highly expressed in the nucleolus as well as other sub-cellular organelles.
Find more information here
Find more information here
Nucleolin is a multi-domain phosphoprotein mapped to human chromosome 2q37. It comprises three domains and a bipartite nuclear localization signal sequence. Nucleolin domains include the highly acidic N-terminal region, a RNA-binding central domain and a glycine, arginine and phenylalanine residues rich C-terminal region.
Spécificité
Anti-Nucleolin-Atto 488 recognizes human, rat, and mouse nucleolin.
Immunogène
synthetic peptide corresponding to amino acid residues 2-17 of human nucleolin conjugated to KLH. The corresponding sequence differs by 4 amino acids in rat and mouse nucleolin, and by one amino acid in the rat homolog protein NRP (nucleolin related protein).
Application
Anti-Nucleolin−Atto 488 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence.
Actions biochimiques/physiologiques
Nucleolin (NCL) interacts with proteins participating in microtubule nucleation. It takes part in the transcription of polymerase I, formation of ribosomes and duplication of centrosomes. NCL is also a transcription regulator. Studies have shown that it is highly expressed in many tumors.
Nucleolin N- and C-terminal domains are involved in protein-protein interaction, whereas the central domain is involved in specific interactions with nucleic acids. Nucleolin exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin′s multiple activities may be regulated by proteolysis and methylation. It also interacts with telomerase andthis interaction is critical for the nucleolar localization of telomerase. Nucleolin might function as a cell surface receptor for lipoproteins, viruses, extracellular matrix, growth factors and other molecules.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Jian-Yu Xu et al.
Asian Pacific journal of cancer prevention : APJCP, 16(8), 3301-3306 (2015-04-30)
Nucleolin (C23) is an important anti-apoptotic protein that is ubiquitously expressed in exponentially growing eukaryotic cells. In order to understand the impact of C23 in radiation therapy, we attempted to investigate the relationship of C23 expression with the radiosensitivity of
Laura Fabre et al.
Biomacromolecules, 23(6), 2302-2314 (2022-05-14)
Nucleolin is a multifunctional protein involved in essential biological processes. To precisely localize it and unravel its different roles in cells, fluorescence imaging is a powerful tool, especially super-resolution techniques. Here, we developed polymer-aptamer probes, both small and bright, adapted
Posterior microphthalmos as a genetically heterogeneous condition that can be allelic to nanophthalmos.
Mohammed A Aldahmesh et al.
Archives of ophthalmology (Chicago, Ill. : 1960), 129(6), 805-807 (2011-06-15)
Marjan M Tajrishi et al.
Communicative & integrative biology, 4(3), 267-275 (2011-10-08)
Nucleolin is a multifunctional phosphoprotein ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. Nucleolin has a bipartite nuclear localization signal sequence and is conserved in animals, plants and yeast. Its levels are correlated with the rate of
Induced expression of nucleolin phosphorylation-deficient mutant confers dominant-negative effect on cell proliferation.
Xiao S
PLoS ONE, 9(10), e109858-e109858 (2014)
Articles
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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