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Key Documents

L8275

Sigma-Aldrich

Lectin from Galanthus nivalis (snowdrop)

lyophilized powder

Synonyme(s) :

Galanthus nivalis lectin, GNL

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About This Item

Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Forme

lyophilized powder

Niveau de qualité

Puissance

<20 μg per mL agglutination activity

Composition

Protein, ≥90% Lowry

Impuretés

salt, essentially free

Température de stockage

2-8°C

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Description générale

Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.

Application

Lectin from Galanthus nivalis (snowdrop) has been used:
  • to coat ELISA plates
  • as reverse transcriptase inhibitor
  • in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses

Actions biochimiques/physiologiques

Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.
GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.

Notes préparatoires

Purified by affinity chromatography according to Kaku.

Remarque sur l'analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Snowdrop lectin.
H Kaku et al.
Methods in enzymology, 179, 327-331 (1989-01-01)
A model for the hepatitis C virus envelope glycoprotein E2
Yagnik AT, et al.
Proteins: Structure, Function, and Bioinformatics, 40, 355-366 (2000)
Jacklyn Johnson et al.
Journal of virology, 94(9) (2020-02-28)
Semen is the primary transmission vehicle for various pathogenic viruses. Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen. However, the unstable nature of human seminal plasma and its toxic effects on cells
Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion
Hogervorst PAM, et al.
Journal of Insect Physiology, 52, 614-624 (2006)
A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects
Trung N, et al
BMC biotechnology, 6, 18-18 (2006)

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