C2010
Concanavalin A from Canavalia ensiformis (Jack bean)
Type IV, lyophilized powder
Synonyme(s) :
Con A
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.32
Produits recommandés
Type
Type IV
Niveau de qualité
Forme
lyophilized powder
Puissance
<64 μg per mL agglutination activity
Impuretés
Carbohydrate, essentially free
salt, essentially free
Température de stockage
−20°C
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Catégories apparentées
Description générale
Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.
Application
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
- as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
- for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
- for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
- for the stimulation of spleen in mitogen assays
Actions biochimiques/physiologiques
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Qualité
Highly purified by specific affinity chromatography
Remarque sur l'analyse
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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