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L7025

Sigma-Aldrich

LB Broth with agar (Lennox)

Tablet microbial growth medium

Synonyme(s) :

LB Agar, LB agar

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.85

Qualité

for molecular biology

Niveau de qualité

200

Stérilité

non-sterile

Forme

tablet

Technique(s)

microbiological culture: suitable

Application(s)

food and beverages
microbiology

Température de stockage

room temp

Adéquation

nonselective for Escherichia coli
nonselective for coliforms

Catégories apparentées

Description générale

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Caractéristiques et avantages

Lennox LB tablet with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Convenient small package to eliminate weighing
  • Fast preparation – no stirring required

Composants

13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents

Reconstitution

Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Informations légales

Zeocin is a registered trademark of Cayla Sarl

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

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LB Broth (Lennox), Tablet microbial growth medium

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L7533

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L3022

Bouillon LB (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

Bouillon LB avec gélose (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

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CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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