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Key Documents

L3272

Sigma-Aldrich

LB Broth with agar (Luria low salt)

Powder microbial growth medium

Synonyme(s) :

Luria Agar Base (Miller’s Modification)

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About This Item

Code UNSPSC :
41106200
Nomenclature NACRES :
NA.85

Qualité

for molecular biology

Niveau de qualité

Stérilité

non-sterile

Forme

powder

Composition

Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

Technique(s)

microbiological culture: suitable

pH

6.8-7.2(3.05% solution)

Application(s)

food and beverages
microbiology

Température de stockage

room temp

Adéquation

nonselective for Escherichia coli
nonselective for coliforms

Description générale

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Application

LB Broth with agar (Luria low salt) has been used:
  • for culturing E. coli DH10B cells.
  • in the isolation of diazotrophic bacteria.
  • for transformation of competent E. coli strain Stbl3 for cloning.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Caractéristiques et avantages

Luria′s low salt LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

Notes préparatoires

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Informations légales

Zeocin is a registered trademark of Cayla Sarl

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth
Grobelak A, et al.
Ecological Engineering, 84, 22-28 (2015)
Increased Expression of a Reporter Plasmid by Crotalid Venom Fraction
Islas AA, et al.
Journal of Animal and Veterinary Advances, 9(17), 2214-2219 (2010)
Difco Manual, 271-271 (1998)
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes

Protocoles

General protocols for growth of competent cells in microbial medium.

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