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L4144

Sigma-Aldrich

Lectine de Phaseolus vulgaris (haricot rouge)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Synonyme(s) :

Agglutinine de Phaseolus vulgaris, PHA

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About This Item

Numéro CE :
232-718-7
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

lyophilized powder

Puissance

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.)
≤1.0 μg per mL leucoagglutination activity
≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

Technique(s)

cell culture | mammalian: suitable

Impuretés

salt, free

Solubilité

DPBS: 5 mg/mL, slightly hazy, colorless

Adéquation

leucocyte agglutination activity tested

Température de stockage

2-8°C

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Description générale

Lectins are carbohydrate-binding proteins, which contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • it has been used as a positive control to treat cells
  • to stimulate cell culture for the analysis of lymphocyte proliferation
  • to stimulate T cells

Actions biochimiques/physiologiques

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.
La PHA correspond à deux espèces moléculaires : l′érythroagglutinine (PHA-E) qui possède une faible activité mitogénique et une forte activité érythroagglutinine, et la leucoagglutinine (PHA-L) qui possède une forte activité mitogénique et leucoagglutinante, mais une activité érythroagglutinante très faible.
La PHA-E n′est pas spécifique au groupe sanguin, mais l′agglutination peut être inhibée par certains oligosaccharides. La PHA-P est la forme protéique de la PHA avant la séparation et la purification de l′érythroagglutinine et de la leucoagglutinine. La PHA-M est la forme mucoprotéique. Les conjugués sont préparés à partir des lectines purifiées correspondantes.

Remarque sur l'analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

SARS-CoV-2 vaccination in rituximab-treated patients: B cells promote humoral immune responses in the presence of T-cell-mediated immunity.
Mrak, et al.
Annals of the Rheumatic Diseases (2021)
Patricia Kaaijk et al.
Scientific reports, 11(1), 13664-13664 (2021-07-03)
Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules
Transfection of FcgammaRIIIa (CD16) Alone Can Be Sufficient To Enable Human alphabetaTCR T Lymphocytes To Mediate Antibody-Dependent Cellular Cytotoxicity
Ollier J, et al.
ImmunoHorizons, 1(5), 63-70 (2017)
Christopher Lai-Hipp et al.
Transfusion, 51(2), 333-337 (2010-08-28)
Cellular substrate (CS) composed of phytohemagglutinin (PHA)-stimulated PBMNCs is required for isolating primary human immunodeficiency virus Type 1 from plasma (PHIV) during early acute infection. The transmitted founder PHIV is neutralization-sensitive and uses chemokine-receptor 5 to infect CD4-positive PBMNCs. Therefore
Haifeng Huang et al.
Acta pharmaceutica Sinica. B, 10(7), 1321-1330 (2020-09-03)
JS001 (toripalimab) is a humanized IgG monoclonal antibody which strongly inhibits programmed cell death protein 1 (PD1). In this study, we used a different iodine isotype (nat/124/125I) to label JS001 probes to target the human PD1 (hPD1) antigen. In vitro
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Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

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