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C0412

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonyme(s) :

Con A

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About This Item

Numéro CAS:
11028-71-0
Numéro CE :
234-258-2
Numéro MDL:
MFCD00071069
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Stérilité

γ-irradiated

Type

Type IV-S

Gamme de produits

BioReagent

Forme

lyophilized powder

Technique(s)

cell culture | mammalian: suitable

Impuretés

salt, essentially free

Solubilité

PBS: 5 mg/mL, slightly hazy
H2O: soluble, slightly hazy

Température de stockage

−20°C

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Catégories apparentées

Application

Concanavalin A from Canavalia ensiformis (jack bean) has been used:
  • to induce spleen blastogenesis
  • to generate ConA blast targets from B6 and lpr spleen cells
  • to stimulate splenic lymphocytes from estrogen-and placebo-treated mice

Actions biochimiques/physiologiques

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Remarque sur l'analyse

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334,H361

Classification des risques

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Chengfang Pang et al.
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Immune responsiveness of splenocytes after chronic daily melatonin administration in male Syrian hamsters
Champney TH, et al.
Immunology Letters, 58(2), 95-100 (1997)
Chengfang Pang et al.
Toxicology letters, 311, 49-57 (2019-04-25)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), the most toxic congener of dioxins, is a persistent and ubiquitous environmental contaminant. Although the immunotoxic effects of TCDD have been reported, the mechanisms underlying these effects are still unclear. In this study, we have determined the toxic
17 beta-Estradiol downregulates interferon regulatory factor-1 in murine splenocytes
Lengi AJ, et al.
Journal of Molecular Endocrinology, 37(3), 421-432 (2006)
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