Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

H2779

Sigma-Aldrich

HistoChoice® Clearing Agent

greener alternative

alternative to toluene and xylene

Synonyme(s) :

dewaxing mixture for paraffin tissue sections

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Niveau de qualité

100

Forme

liquid

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Couleur

colorless

Densité

0.760-0.820 g/mL

Application(s)

hematology
histology

Autre catégorie plus écologique

, Aligned

Température de stockage

room temp

Description générale

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites, and nucleic acid sites. It is a non-toxic, non-cross-linking fixative formulated to replace formaldehyde-based fixation. This formulation in non-citrus, contains no mercury, formaldehyde, or glutaraldehyde.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of formalin for tissue processing.

Application

HistoChoice® Clearing Agent has the following applications:
  • It is suitable as an alternative to toluene and xylene for dewaxing paraffin tissue sections. Prepared slides are suitable for antibody probing applications and in situ hybridizations.
  • It has been employed in a study to explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation.
  • It has been used to develop a novel method to improve sensitivity for the localization of miRNA and their target transcripts in plants.
  • It has been used to study the correlation of cytoplasmic ADP-ribosylation levels with markers of patient outcome in human cancers.

Actions biochimiques/physiologiques

Fixation with HistoChoice® works well in immunohistology applications where its non-cross-linking traits are conducive to epitope retrieval and identification by primary antibodies to extracellular matrix components.

Caractéristiques et avantages

  • Non-toxic, non-carcinogenic, and odorless.
  • Non-flammable.
  • Preserves native antigenic and nucleic acid sites.
  • Compatible with many organic mounting media.
  • Dissolves wax faster and evaporates slower than xylene.
  • Can be used in automated processors.
  • Tissues retain their original structure.

Informations légales

HistoChoice is a registered trademark of Amresco, Inc.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

142.0 °F

Point d'éclair (°C)

61.1 °C

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Sathyanesan Samuel Newton et al.
Brain research. Brain research protocols, 9(3), 214-219 (2002-07-13)
A method is described to perform combined immunohistochemistry and in situ hybridization in mouse brain sections. The protocol is specific to sections mounted on glass slides. In contrast to earlier methods that require either paraffin embedding or perfusion of the
Synchrotron Time-Lapse Imaging of Lignocellulosic Biomass Hydrolysis: Tracking Enzyme Localization by Protein Autofluorescence and Biochemical Modification of Cell Walls by Microfluidic Infrared Microspectroscopy
Devaux, et al.
Frontiers in Plant Science (2018)
Cytoplasmic ADP-ribosylation levels correlate with markers of patient outcome in distinct human cancers
Aimi, et al.
Modern Pathology (2021)
Anita S Patel et al.
The Journal of investigative dermatology, 128(12), 2888-2893 (2008-06-13)
An increasing number of studies report that genus beta human papillomaviruses (HPVs) are associated with skin cancer, with suggestions of specificity for squamous cell carcinoma (SCC) of the skin. We have conducted a systematic examination of HPV DNA in tumors
Marie-Françoise Devaux et al.
Frontiers in plant science, 9, 200-200 (2018-03-09)
Tracking enzyme localization and following the local biochemical modification of the substrate should help explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation. Time-lapse studies using conventional imaging require enzyme labeling and following the biochemical modifications of biopolymers
Afficher tous les articles scientifiques apparentés

Contenu apparenté

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Preparation & Staining of 3D Printed Tissues & Scaffolds

Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique