F8924

Fibroblast Growth Factor-10 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : FGF-10
• Numéro MDL: MFCD01867039
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: >97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 28 ng/mL
• Poids mol.: 19 kDa by SDS-PAGE
• Conditionnement: pkg of 25 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: O15520
• Température de stockage: −20°C
• Informations sur le gène: human ... FGF10(2255)

Description

Description générale

FGF10 (fibroblast growth factor 10) is a member of the FGF protein family, which is a mitogen of epithelial origin and has a paracrine activity. It is produced by mesenchymal lineage cells, and acts as a ligand for FGFR, most notably FGFR2b, which is expressed by epithelial cells.

Application

Fibroblast Growth Factor-10 human is suitable for culturing of HUVEC cell line (human umbilical vein endothelial cells) to determine the anti-angiogenic properties of sFLT-1 (soluble fms-related tyrosine kinase-1).

Actions biochimiques/physiologiques

FGF10 (fibroblast growth factor 10) is involved in cell growth, morphogenesis and embryogenesis. It plays an essential role in initiating the formation of limb bud, lung morphogenesis and brain formation during embryogenesis. It acts as a mitogen, where it facilitates the regeneration of epithelia post-bladder urothelial injury. Thus, it might have future applications during bladder transplantation. Studies in Japanese population show that polymosphisms- rs339501, rs12517396, and rs10462070 are linked with severe myopia, and might act as susceptibility factors for the same. In lung cancer cells, FGFR4 reduces the stem cell/self-renewal property of cancer cells, and makes them more susceptible to chemotherapy.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS, 50 mM sodium sulfate, 1 mM DTT, 0.5 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Remarque sur l'analyse

The biological activity is measured by its ability to stimulate the proliferation of a monkey epithelial cell line 4MBr-5.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable