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Key Documents

F8264

Sigma-Aldrich

Anti-Mouse IgG (γ-chain specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Conjugué

FITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct immunofluorescence: 1:64

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (γ-chain specific)-FITC antibody is specific for is specific for mouse IgG when tested against purified mouse IgA, IgG (all subclasses), and mouse IgM. Goat anti-mouse IgG is isolated by affinity isolation and conjugated to Fluorescein Isothiocyanate (FITC), isomer I.

Immunogène

Purified mouse IgG

Application

Anti-Mouse IgG (γ-chain specific)-FITC antibody may be used for immunofluorescence of mouse spleen cells at a working antibody dilution of 1:64. For ELISA using mice sera, antibody dilution of 1:50 was used. The antibody was also used in anti-nucear antibody detection using mouse sera and for labeling Saimiri brain endothelial cells for exhaustive photon reassignment microscopy
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Consulter la Bibliothèque de documents

C M Shih et al.
Journal of clinical microbiology, 33(12), 3164-3168 (1995-12-01)
We determined whether the infectivity of the Lyme disease spirochete (Borrelia burgdorferi) to vector ticks varies with the duration of infection in laboratory mice. Thus, noninfected nymphal deer ticks were permitted to feed on two strains of early (2 months
C M Shih et al.
Journal of clinical microbiology, 34(9), 2297-2299 (1996-09-01)
We determined whether the span of infectivity of Lyme disease spirochetes (Borrelia burgdorferi) to vector ticks varies with the mode of infection in laboratory mice. Noninfected larval deer ticks were permitted to feed on two strains of spirochete-infected mice that
Anna A Ogienko et al.
International journal of molecular sciences, 25(2) (2024-01-27)
The nucleolus is a significant nuclear organelle that is primarily known for its role in ribosome biogenesis. However, emerging evidence suggests that the nucleolus may have additional functions. Particularly, it is involved in the organization of the three-dimensional structure of
V P Chekhonin et al.
Drug delivery, 12(1), 1-6 (2005-04-02)
Polyethylene glycol (PEG)ylated (stealth) immunoliposomes directed against human gliofibrillary acidic protein (GFAP) were prepared by coupling the thiolated monoclonal anti-GFAP antibodies with a maleimide derivative of phosphatidyl ethanolamine of the liposomal membrane. Experiments with cell cultures demonstrated specific and competitive
Anton Strunov et al.
BMC biology, 16(1), 68-68 (2018-06-17)
S2 cells are one of the most widely used Drosophila melanogaster cell lines. A series of studies has shown that they are particularly suitable for RNAi-based screens aimed at the dissection of cellular pathways, including those controlling cell shape and

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