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D7058

Sigma-Aldrich

DirectLoad™ Wide Range DNA Marker

ready-to-use marker for DNA electrophoresis

Synonyme(s) :

DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder, wide range ladder for gel electrophoresis, wide range marker for gel electrophoresis

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About This Item

Code UNSPSC :
41105335
Nomenclature NACRES :
NA.25

Niveau de qualité

100

Forme

liquid

Utilisation

100 uses

Adéquation

suitable for electrophoresis (DNA)

Température de stockage

−20°C

Description générale

Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Caractéristiques et avantages

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Composants

Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.

Autres remarques

Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.

Informations légales

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Protocoles

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

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Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

End Point PCR Protocol for Long and Accurate DNA Amplification

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

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