11336045001

DNA Molecular Weight Marker VIII

pkg of 50 μg (in 200 μl), solution

• Synonyme(s) : DNA marker
• Code UNSPSC : 41105335

Propriétés

• Forme: solution
• Niveau de qualité: 100
• Conditionnement: pkg of 50 μg (in 200 μl)
• Fabricant/nom de marque: Roche
• Concentration: 250 μg/mL
• Couleur: colorless
• Solubilité: water: miscible
• Température de stockage: −20°C

Description

Description générale

DNA Molecular Weight Marker VIII contains a mixture of 17 DNA fragments.
The product is also available as DNA Molecular Weight Marker VIII, Digoxigenin-labeled.
Size range: 19 to 1114 bp

Application

DNA Molecular Weight Marker VIII is used for the size determination of DNA in agarose gels by:

• Restriction digests
• PCR
• RT-PCR

Note: Use this marker to size DNA fragments between 19 and 1,114 bp.
This marker is also available in DIG-labeled form (DNA Molecular Weight Marker VIII, DIG-labeled).

Caractéristiques et avantages

Fragment mixture prepared by cleavage of pUCBM21 DNA with Hpa II, and pUCBM21 DNA with Dra I and Hind III.
Contents
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Séquence

The mixture contains 17 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 19, 26, 34/34, 37, 67, 110, 124, 147, 190, 242, 320, 404, 489, 501, 692, 900, and 1114 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Définition de l'unité

50 μg = 1A₂₆₀ unit

Notes préparatoires

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Use this marker to size DNA fragments between 19 and 1,114 bp.
This marker is also available in DIG-labeled form (DNA Molecular Weight Marker VIII, DIG-labeled).

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash