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D3687

Sigma-Aldrich

DirectLoad™ PCR 100 bp Low Ladder

ready-to-use marker for DNA electrophoresis

Synonyme(s) :

100bp ladder for gel electrophoresis, 100bp maker, DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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About This Item

Code UNSPSC :
41105335
Nomenclature NACRES :
NA.31

Qualité

for molecular biology

Niveau de qualité

200

Forme

liquid

Utilisation

75 uses

Adéquation

suitable for electrophoresis (DNA)

Température de stockage

−20°C

Description générale

Sigma′s DirectLoad™ PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.

Application

Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Caractéristiques et avantages

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Composants

Sigma′s DirectLoad™ PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Autres remarques

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Informations légales

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Protocoles

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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