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Key Documents

D4818

Sigma-Aldrich

Dispase I

protease

Synonyme(s) :

Protéase from Bacillus polymyxa

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About This Item

Numéro CAS:
42613-33-2
Numéro CE :
255-914-4
Numéro MDL:
MFCD02253017
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Niveau de qualité

200

Forme

lyophilized solid

Activité spécifique

≥10 unit/mg solid

Technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

Température de stockage

2-8°C

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Application

Dispase I has been used in a study to assess the effect of amniotic membrane on wound size in the early stages of the healing process. Dispase I has also been used in a study to investigate a dityrosine-based substrate for a protease assay.
Dispase I has been used in lung digestion and processing for flow staining, as well as for CD4 cell isolation in mice. The enzyme has also been used to digest excised wounds and a small amount of surrounding skin for the detection of GFP+ (green fluorescence protein) cells. This study to investigated the effect of differentiation and angiogenesis of bone marrow-derived mesenchymal stem cells on wound healing. It has also been used to remove the epidermis during the isolation of dermal fibroblasts from mice.

Suitable for use in preparation of single cell suspension for sequencing.

Actions biochimiques/physiologiques

Dispase I is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. It can also separate intact epithelial sheets in culture from the substratum. The enzyme preserves the viability of the epithelial cells while cleaving the basement membrane zone region. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. It hydrolyzes N-terminal peptide bonds of non-polar amino acid residues. It preferentially attacks denatured and intercellular proteins with exposed hydrophobic amino acid residues. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+ and other heavy metals inhibit the enzyme activity. The enzyme contains 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. The enzyme is not inhibited by serum.

Définition de l'unité

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Forme physique

lyophilized powder containing calcium acetate

Informations légales

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Alizée Vercauteren Drubbel et al.
Cell stem cell, 28(8), 1411-1427 (2021-04-22)
Columnar metaplasia of the esophagus is the main risk factor for esophageal adenocarcinoma. There is a lack of evidence to demonstrate that esophageal progenitors can be the source of columnar metaplasia. In this study, using transgenic mouse models, lineage tracing
Yaojiong Wu et al.
Stem cells (Dayton, Ohio), 25(10), 2648-2659 (2007-07-07)
Although chronic wounds are common, treatment for these disabling conditions remains limited and largely ineffective. In this study, we examined the benefit of bone marrow-derived mesenchymal stem cells (BM-MSCs) in wound healing. Using an excisional wound splinting model, we showed
Liwen Chen et al.
PloS one, 4(9), e7119-e7119 (2009-09-23)
Studies have shown that allogeneic (allo-) bone marrow derived mesenchymal stem cells (BM-MSCs) may enhance tissue repair/regeneration. However, recent studies suggest that immune rejection may occur to allo-MSCs leading to reduced engraftment. In this study, we compared allo-BM-MSCs with syngeneic
Marco Tatullo et al.
Materials (Basel, Switzerland), 12(4) (2019-02-20)
Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting
Some properties of a protease from Bacillus polymyxa.
P J Griffin et al.
The Biochemical journal, 125(4), 109P-109P (1971-12-01)
Afficher tous les articles scientifiques apparentés

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Collagenase Blends for Gentle Tissue Dissociation

Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.

Collagenase Blends for Gentle Tissue Dissociation

Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.

Collagenase Blends for Gentle Tissue Dissociation

Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.

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