CS1070
Transglutaminase Assay Kit
sufficient for assays in two 96-well plates
Synonyme(s) :
TGases Assay Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.28
Produits recommandés
Niveau de qualité
Conditions d'expédition
dry ice
Température de stockage
−20°C
Informations sur le gène
human ... TGM1(7051) , TGM2(7052) , TGM3(7053) , TGM4(7047) , TGM5(9333) , TGM6(343641) , TGM7(116179)
Description générale
The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.
Application
The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.
Actions biochimiques/physiologiques
Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing. Deregulation of the enzyme activity contributes to a number of human diseases.
Autres remarques
The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
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Les clients ont également consulté
Bovine aortic endothelial cell transglutaminase. Enzyme characterization and regulation of activity.
G Korner et al.
The Biochemical journal, 262(2), 633-641 (1989-09-01)
Bovine aortic endothelial cells contain Ca2+-dependent tissue-type transglutaminase. Its activity in these cells was high, with apparent Km and Vmax. values with respect to putrescine of 0.203 mM and 18.5 nmol/min per mg of protein, and its activity was inhibited
Transglutaminases and their substrates.
Francesco Facchiano et al.
Progress in experimental tumor research, 38, 37-57 (2005-03-05)
Mechanism of action of guinea pig liver transglutaminase. VII. Chemical and stereochemical aspects of substrate binding and catalysis.
S I Chung et al.
The Journal of biological chemistry, 245(23), 6424-6435 (1970-12-10)
Claudia Damaris Müller et al.
Scientific reports, 12(1), 13326-13326 (2022-08-04)
Transglutaminases (TGs) catalyze the covalent crosslinking of proteins via isopeptide bonds. The most prominent isoform, TG2, is associated with physiological processes such as extracellular matrix (ECM) stabilization and plays a crucial role in the pathogenesis of e.g. fibrotic diseases, cancer
Daniel Rittschof et al.
PloS one, 6(2), e16487-e16487 (2011-03-08)
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