SNL 76/7 Cell Line from mouse

7032801, mouse esophagus, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: SNL 76/7 Cell Line from mouse, 07032801
• Source biologique: mouse esophagus
• Description: Mouse SIM strain embryonic fibroblast
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse SIM strain embryonic fibroblast

Description de la lignée cellulaire

SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.

Milieu de culture

Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C.

Autres remarques

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