RAW 264.7 Cell Line murine

91062702, mouse blood, Macrophage

• Synonyme(s) : RAW264 7 Cells, RAW264.7 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: RAW 264.7 Cell Line murine, Macrophage from blood, 91062702
• Source biologique: mouse blood
• Mode de croissance: Semi-adherent
• Caryotype: Not specified
• Morphologie: Macrophage
• Produits: Lysozyme
• Récepteurs: Immunoglobulin, complement
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse monocyte macrophage

Description de la lignée cellulaire

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

Application

Metabolic studies

RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin. It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.

Milieu de culture

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Autres remarques

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