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Key Documents

NIH 3T3 Cell Line murine

93061524, mouse embryo, Fibroblast

Synonyme(s) :

3T3 Cells, Mouse 3T3 Cells, NIH/3T3 Cells, NIH3T3 Cells, Swiss 3T3 Cells, Swiss3T3 Cells

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About This Item

Code UNSPSC :
41106514

product name

NIH 3T3 Cell Line murine, 93061524

Source biologique

mouse embryo

Description

Embryonic fibroblast

Mode de croissance

Adherent

Caryotype

Not specified

Morphologie

Fibroblast

Produits

Not specified

Récepteurs

Not specified

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

dry ice

Température de stockage

−196°C

Origine de la lignée cellulaire

Mouse Swiss NIH embryo

Description de la lignée cellulaire

Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.

Application

DNA transfection studies
NIH 3T3 Cell Line has been used to:
  • study calcium-mediated actin reset (CaAR) in response to physiological changes
  • study the effects of activation of the transient receptor potential vanilloid 3 channel (TRPV3) on adipocyte differentiation
  • determine the viral titer of the retroviral construct -ST40hTNFα

Milieu de culture

DMEM + 2mM Glutamine + 10% Calf Serum (CS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Do not allow culture to become fully confluent; the use of fetal calf serum is not recommended.

Autres remarques

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Certificats d'analyse (COA)

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Articles

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Protocoles

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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