B8503

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

chromogenic, ≥99% (HPLC), powder

• Synonyme(s) : 5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt, BCIP^® p-toluidine salt, X-phosphate p-toluidine salt
• Formule empirique (notation de Hill): C₈H₆BrClNO₄P · C₇H₉N
• Numéro CAS: 6578-06-9
• Poids moléculaire : 433.62
• Numéro CE : 229-506-1
• Numéro MDL: MFCD00040636
• Code UNSPSC : 12352204
• ID de substance PubChem : 57653963
• Nomenclature NACRES : NA.83

Propriétés

• product name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, ≥99% (HPLC)
• Niveau de qualité: 200
• Pureté: ≥99% (HPLC)
• Forme: powder
• Solubilité: DMF: 20 mg/mL, clear to slightly hazy, yellow
• Température de stockage: −20°C
• Chaîne SMILES: Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
• Clé InChI: QEIFSLUFHRCVQL-UHFFFAOYSA-N

Description

Description générale

5-bromo-4-chloro-3-indolyl phosphate (BCIP) is an histochemical phosphatase stain.

Application

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt has been used in in situ hybridization.

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Actions biochimiques/physiologiques

5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphatase activity in solution.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrats

Histochemical substrate for alkaline phosphatase.

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves