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B6777

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

BioReagent, ≥99%, powder, suitable for molecular biology

Synonyme(s) :

5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt, BCIP® p-toluidine salt, X-phosphate p-toluidine salt

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About This Item

Formule empirique (notation de Hill):
C8H6BrClNO4P · C7H9N
Numéro CAS:
6578-06-9
Poids moléculaire :
433.62
Numéro CE :
229-506-1
Numéro MDL:
MFCD00040636
Code UNSPSC :
12352204
ID de substance PubChem :
24891823
Nomenclature NACRES :
NA.83

product name

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BioReagent, for molecular biology, powder, ≥99%

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Pureté

≥99%

Forme

powder

Solubilité

DMF: 20 mg/mL
H2O: insoluble

Activité étrangère

Protease, none detected

Température de stockage

−20°C

Chaîne SMILES 

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

Clé InChI

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrats

Histochemical substrate for alkaline phosphatase.

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

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Histochemistry and cell biology, 135(3), 263-280 (2011-02-25)
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Kum C Hiong et al.
The Journal of experimental biology, 218(Pt 23), 3717-3728 (2015-10-10)
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Daniel Steil et al.
Journal of lipid research, 56(12), 2322-2336 (2015-10-16)
Shiga toxins (Stxs) are produced by enterohemorrhagic Escherichia coli (EHEC), which cause human infections with an often fatal outcome. Vero cell lines, derived from African green monkey kidney, represent the gold standard for determining the cytotoxic effects of Stxs. Despite

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