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11681451001

Roche

NBT/BCIP Stock Solution

Synonyme(s) :

5-bromo-4-chloro-3-indoyl phosphate, BCIP

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About This Item

Code UNSPSC :
12352204

Forme

solution

Niveau de qualité

100

Poids mol.

817.7

Conditionnement

pkg of 8 mL

Fabricant/nom de marque

Roche

Température de stockage

2-8°C

Description générale

The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

Evaluation: visually

Application

  • The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
  • Northern blot
  • Western blot
  • Colony and plaque lifts
  • In situ hybridization
  • and for applications in immunohistochemistry and immunocytochemistry.
Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

Forme physique

Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.

Notes préparatoires

Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use.
  • For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
  • For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Note: Do not include MgCl2 in the detection buffer as this may lead to spotty background on the membrane after the detection procedure. Insoluble in water.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

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João L Q Durigan et al.
Muscle & nerve, 49(1), 120-128 (2013-04-30)
Neuromuscular electrical stimulation (NMES) is used to improve quadriceps mass after anterior cruciate ligament (ACL) injury. We studied the effect of NMES on mRNA levels of atrophy genes in the quadriceps muscle of rats after ACL transection. mRNA levels of
Jaroslav Fabik et al.
Biology open, 9(6) (2020-07-04)
Cranial neural crest cells (cNCCs) originate in the anterior neural tube and populate pharyngeal arches in which they contribute to formation of bone and cartilage. This cell population also provides molecular signals for the development of tissues of non-neural crest
Morgan Haugen et al.
Cold Spring Harbor protocols, 2010(10), pdb-pdb (2010-10-05)
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have
Garth R Ilsley et al.
Scientific reports, 10(1), 4961-4961 (2020-03-20)
Single-cell RNA-seq has been established as a reliable and accessible technique enabling new types of analyses, such as identifying cell types and studying spatial and temporal gene expression variation and change at single-cell resolution. Recently, single-cell RNA-seq has been applied
David Lagman et al.
PloS one, 10(3), e0121330-e0121330 (2015-03-26)
Gene duplications provide raw materials that can be selected for functional adaptations by evolutionary mechanisms. We describe here the results of 350 million years of evolution of three functionally related gene families: the alpha, beta and gamma subunits of transducins
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