A6014

Acridine Orange hemi(zinc chloride) salt

For nucleic acid staining in cells or gels

• Synonyme(s) : 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
• Formule linéaire : C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
• Numéro CAS: 10127-02-3
• Poids moléculaire : 369.96
• Numéro C.I. (Colour Index): 46005
• Numéro Beilstein : 3734978
• Numéro CE : 233-353-6
• Numéro MDL: MFCD00081043
• Code UNSPSC : 12171500
• ID de substance PubChem : 24891048
• Nomenclature NACRES : NA.52

Propriétés

• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: powder
• Composition: Dye content, ~80%
• Technique(s): nucleic acid detection: suitable
• Solubilité: ethanol: 2 mg/mL; 4 mg/mL (2-methoxyethanol (EGME)); water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
• Adéquation: suitable for flow cytometry; suitable for microscopy
• Température de stockage: room temp
• Chaîne SMILES: Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
• InChI: 1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
• Clé InChI: RAHGLSRJKRXOSY-UHFFFAOYSA-L

Description

Description générale

Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

Application

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Suitable for

• detection of nucleic acids separated by gel electrophoresis
• fluorescence and epifluorescence microscopy
• analysis of mitochondria and lysosomes by flow cytometry
• DNA staining in apoptosis studies

Caractéristiques et avantages

• 120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
• differential staining of single- and double-stranded polynucleotides

Principe

Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)