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Key Documents

A0793

Sigma-Aldrich

Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Rabbit Anti-Dog IgG (whole molecule)–AP

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

dog

Technique(s)

direct ELISA: 1:6,000
dot blot: 1:4,000
western blot (chemiluminescent): 1:4,000

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. In dogs four subclasses of IgG (IgG1 -IgG4) are present.
Dog IgGs are glycoprotein antibodies that regulate several immunological responses. Canine IgGs can be used as markers for detecting immune-mediated haemolytic anaemia, adverse food reactions and also for assessing the risk of L. infantum transmission in dogs.

Spécificité

Anti-Dog IgG (whole molecule) antibody is specific for IgG in dogs when tested against normal dog serum and dog IgG by double-diffusion and immunoelectrophoretic assays prior to conjugation.

Immunogène

IgG isolated from pooled normal dog serum

Application

Anti-Dog IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA), and immunoblotting.

Actions biochimiques/physiologiques

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Delivery of recombinant gene product to canine brain with the use of microencapsulation
Barsoum SC, et al.
The Journal of Laboratory and Clinical Medicine, 142(6), 399-413 (2003)
Comparative evaluation of several methods for Canine Visceral Leishmaniasis diagnosis
Silva DT da, et al.
Revista Brasileira de Parasitologia veterinaria = Brazilian Journal of Veterinary Parasitology : Org?o Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 23(2), 179-186 (2014)
C Sriwichai et al.
The Journal of small animal practice, 52(9), 476-483 (2011-09-08)
Canine allo- or autoantibodies are clinically important, but attachment of these immunoglobulin G (IgG) antibodies does not produce observable haemagglutination. Antibody to canine globulins is required to demonstrate sensitisation of red blood cells. Commercial reagents are available, but these often
Michaela Vlkova et al.
PLoS neglected tropical diseases, 5(10), e1344-e1344 (2011-10-25)
Phlebotomine sand flies are blood-sucking insects that can transmit Leishmania parasites. Hosts bitten by sand flies develop an immune response against sand fly salivary antigens. Specific anti-saliva IgG indicate the exposure to the vector and may also help to estimate
Joseph A Impellizeri et al.
Journal of translational medicine, 16(1), 349-349 (2018-12-13)
we have recently shown that Tel-eVax, a genetic vaccine targeting dog telomerase (dTERT) and based on Adenovirus (Ad)/DNA Electro-Gene-Transfer (DNA-EGT) technology can induce strong immune response and increase overall survival (OS) of dogs affected by multicentric Diffuse Large B cell

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