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A0480

Sigma-Aldrich

Anti-Actin (plant) antibody, Mouse monoclonal

clone 10-B3 (MAbGPa), purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Actin (plant) antibody produced in mouse

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About This Item

Numéro MDL:
MFCD00145889
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

10-B3 (MAbGPa), monoclonal

Poids mol.

antigen ~45 kDa

Espèces réactives

plant

Conditionnement

antibody small pack of 25 μL

Technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using corn leaves extracts

Isotype

IgG2b

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Arabidopsis thaliana ... ACT8(841347)

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Description générale

Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Monoclonal Anti-Actin (plant) antibody recognizes actin in several plant species including Dictyostelium. In Arabidopsis, the antibody recognizes all eight actins isoforms (ACT1, 2, 3, 4, 7, 8, 11 and 12).
Monoclonal Anti-Actin (plant) (mouse IgG2b isotype) is derived from the hybridoma 10-B3 (MabGPa) produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with ACT8 protein. In Arabidopsis, actin is divided into two major phylogenetic classes namely reproductive and vegetative class.

Immunogène

ACT8 protein.

Application

Monoclonal Anti-Actin (plant) antibody produced in mouse has been used in:
  • two-layer phos-tag polyacrylamide gel electrophoresis (PAGE)
  • immunohistochemistry and enzyme-linked immunosorbent assay (ELISA).
  • immunoblotting

Actions biochimiques/physiologiques

In Arabidopsis ACT7 isovariant is essential for normal phytohormone response to hormones in different organs. Mutation in the actin (ACT)1 gene is associated with the development of dwarfism in plants in addition to delayed flowering, highly reduced organ size and varied branching pattern of the leaf trichomes and inflorescence stem. In plants, there is a developmental switch in the modulation of actin isovariants throughout cell differentiation and maturation phase.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Andrés Ritter et al.
Nature communications, 8, 15235-15235 (2017-05-12)
Most living organisms developed systems to efficiently time environmental changes. The plant-clock acts in coordination with external signals to generate output responses determining seasonal growth and flowering time. Here, we show that two Arabidopsis thaliana transcription factors, FAR1 RELATED SEQUENCE
Functional Nonequivalency of Actin Isovariants in
Kandasamy, et al.
Molecular Biology of the Cell, 13(1), 251-251 (2002)
Jodi L Stewart et al.
PloS one, 6(5), e19894-e19894 (2011-06-01)
As photoautotrophs, plants can use both the form and amount of fixed carbon as a measure of the light environment. In this study, we used a variety of approaches to elucidate the role of exogenous sucrose in modifying seedling growth
Y Q An et al.
The Plant journal : for cell and molecular biology, 10(1), 107-121 (1996-07-01)
Arabidopsis has a complex and ancient actin gene family encoding six divergent subclasses of proteins. One subclass is represented by ACT2 and ACT8, which encode nearly identical proteins. These two genes differ significantly in flanking and intron sequences and in
An Endogenous Carbon-Sensing Pathway Triggers Increased Auxin Flux and Hypocotyl Elongation
Lilley JL, et al.
Plant Physiology, 160(4), 2261-2270 (2012)
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