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67309

Millipore

Kovac′s reagent for indoles

suitable for microbiology

Synonyme(s) :

4-(Dimethylamino)benzaldehyde solution

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About This Item

Formule empirique (notation de Hill):
C9H11NO
Numéro CAS:
100-10-7
Poids moléculaire :
149.19
Numéro Beilstein :
4132845
Numéro MDL:
MFCD00003381
Code UNSPSC :
41171621
ID de substance PubChem :
329760541
Nomenclature NACRES :
NA.85

Agence

according to ISO 16654:2001

Niveau de qualité

200

Gamme de produits

BioChemika

Durée de conservation

limited shelf life, expiry date on the label

Composition

4-(dimethlyamino)benzaldehyde, 50 g/L
hydrochloric acid, 240 g/L
isoamylic alcohol, 710 g/L

Technique(s)

microbe id | specific enzyme detection: suitable

Application(s)

agriculture
clinical testing
environmental
food and beverages
microbiology

Température de stockage

2-8°C

Adéquation

Escherichia coli
coliforms

Chaîne SMILES 

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

Clé InChI

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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Description générale

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

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Réf. du produit
Description
Tarif

Pictogrammes

FlameCorrosionExclamation mark
GHS02,GHS05,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

109.4 °F

Point d'éclair (°C)

43 °C

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCL5138
BCCK4337
BCCH8824
BCCG3914
BCCF1994

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Rita R. Colwell, R. Grigorova
Methods in Microbiology, 19, 27-27 (1987)
S. Harisha
An Introduction to Practical Biotechnology, 193-193 (2005)
Patrick R Porubsky et al.
Archives of biochemistry and biophysics, 475(1), 14-17 (2008-04-22)
Cytochrome P450 2A13 (CYP2A13) is a lung specific enzyme known to activate the potent tobacco procarcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) into two carcinogenic metabolites. CYP2A13 has been crystallized and X-ray diffraction experiments illuminated the structure of this enzyme, but with an unknown
R W Trepeta et al.
Journal of clinical microbiology, 19(2), 172-174 (1984-02-01)
Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and
Jun-Min Guo et al.
Journal of agricultural and food chemistry, 58(11), 6556-6561 (2010-05-15)
A simple colorimetric method for the differentiation of indoleacetic acid (IAA) and indolebutyric acid (IBA) in plant samples is described. The color change is based upon the reaction between the auxins and p-(dimethylamino)benzaldehyde (PDAB, Ehrlich reagent) following the electrophilic substitution
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