Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

1.11350

Millipore

Bactident™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

Synonyme(s) :

4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41106200
Nomenclature NACRES :
NA.84

Niveau de qualité

100

Description

Bactident™ E. coli 1.13303

Forme

liquid (ready-to-use)

Durée de conservation

210 days

Caractéristiques

ready-to-use

Composition

(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)

Conditionnement

pkg of (1 x 30 ml, Plastic bottle)

Technique(s)

microbe id | specific enzyme detection: suitable

pH

<1 (20 °C in H2O)

Température de transition

flash point 36 °C

Densité

0.92 g/cm3 at 20 °C

Application(s)

environmental
food and beverages

Température de stockage

2-8°C

Adéquation

Enterobacter spp.
Escherichia coli

Description générale

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Application

Bactident™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

Liaison

Replaces: 1.11350.0007

Remarque sur l'analyse

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

Informations légales

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

FlameCorrosionExclamation mark
GHS02,GHS05,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

96.8 °F

Point d'éclair (°C)

36 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Cultivation of Shigella

Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions. 

Cultivation of Shigella

Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions. 

Cultivation of Shigella

Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions. 

Cultivation of Shigella

Dehydrated culture media for cultivating Shigella come in granules that dissolve in water. Granulation of the media ensures homogenous distribution, which avoids clumping even in warm and humid conditions. 

Afficher tout

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique