BGALD-RO

β-Galactose Dehydrogenase

from Pseudomonas fluorescens

• Synonyme(s) : D-galactose dehydrogenase, D-galactose:NAD+ 1-oxidoreductase
• Numéro de classification (Commission des enzymes): 1.1.1.48 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: Pseudomonas fluorescens
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Forme: suspension
• Activité spécifique: ~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)
• Poids mol.: 64,000 Da
• Conditionnement: pkg of 1 mL (10104973001 [1 mg]); pkg of 1 mL (10104981001 [5 mg])
• Fabricant/nom de marque: Roche
• Technique(s): activity assay: suitable
• Couleur: white
• pH optimal: 8.4 (0.1 M in Tris buffer)
• Plage de pH: 4.6-9.2
• Solubilité: water: soluble
• Adéquation: suitable for enzyme test
• Numéro d'accès UniProt: (T1WEA6 · T1WEA6_PSEFL)
• Activité étrangère: ADH <0.01%; LDH <0.5%; NADH oxidase <0.1%; galactosidase <0.01%
• Température de stockage: 2-8°C

Description

Description générale

β-Galactose Dehydrogenase plays a crucial role in galactose metabolism by catalyzing the pyridine-nucleotide-dependent dehydrogenation of β-D-galactopyranose to yield NADH and D-galactono-l,5-1actone. D-galactose dehydrogenase from P. fluorescens is a member of the oxidoreductases family. It is composed of two identical subunits and has a molecular weight of 64,000. Additionally, the enzyme contains two binding sites.

Spécificité

Galactose dehydrogenase will oxidize D-galactose [K_m = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (K_m = 0.24 mM; relative rate = 1.0) or NADP (K_m = 2.3 mM; relative rate = 0.32).

Application

β-Galactose Dehydrogenase has been used as a reporter enzyme in neuraminidase (NA) kinetic parameters for the natural substrates 6′-Sialyl-N-acetyllactosamine (6SLN) and 3′-Sialyl-N-acetyllactosamine (3SLN).

Qualité

Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH

Séquence

Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).

Définition de l'unité

One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 °C and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.

Forme physique

Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available