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PC138

Sigma-Aldrich

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-Nuclear Factor ΚB

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

rabbit

Niveau de qualité

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Espèces réactives

human

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Rabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
Recognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Immunogène

Human
a synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH

Application

ELISA (see comments)

Gel Shift (see comments)

Immunoblotting (1:500, chemiluminescence)

Immunoprecipitation (see comments)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 5 mM sodium phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Positive Control
Daudi cells

Autres remarques

Kolberg, R., 1997. J. NIH Research9, 29.
Van Antwerp, D.J. et al. 1996. Science274, 787.
Beg, A.A., et al. 1995. Nature376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol.12, 141.
Wulczyn, F.G., et al. 1992. Nature358, 597.
This antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Chiara Riganti et al.
American journal of respiratory cell and molecular biology, 36(6), 746-756 (2007-02-27)
We have observed that in three human malignant mesothelioma cell lines, crocidolite asbestos induced the activation of the transcription factor NF-kappaB and the synthesis of nitric oxide (NO) by inhibiting the RhoA signaling pathway. The incubation with crocidolite decreased the
Cielo Mae D Marquez et al.
Asian Pacific journal of cancer prevention : APJCP, 21(8), 2453-2461 (2020-08-29)
The chemotherapy drug doxorubicin (DOX) is effective in treating many types of cancers. However, due to its pro-inflammatory and cardiotoxic side effects, other remedies have also been explored as alternative treatments. The plant Alangium longiflorum was reported to contain cytotoxic
Gaoxiang Huang et al.
Cellular & molecular immunology, 14(3), 265-275 (2015-09-22)
Immune cells, particularly macrophages, play critical roles in the hypoxia-induced inflammatory response. The small GTPase RhoB is usually rapidly induced by a variety of stimuli and has been described as an important regulator of cytoskeletal organization and vesicle and membrane
Raquel Fernández-Ginés et al.
Redox biology, 55, 102396-102396 (2022-07-16)
It is widely accepted that activating the transcription factor NRF2 will blast the physiological anti-inflammatory mechanisms, which will help combat pathologic inflammation. Much effort is being put in inhibiting the main NRF2 repressor, KEAP1, with either electrophilic small molecules or

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