MMYOMAG-74K

MILLIPLEX^® Mouse Myokine Magnetic Bead Panel

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple myokine and cytokine biomarkers in mouse serum and plasma samples.

• Code UNSPSC : 12161503
• eCl@ss : 32161000
• Nomenclature NACRES : NA.47

Propriétés

• Niveau de qualité: 200
• Espèces réactives: mouse
• Fabricant/nom de marque: Milliplex^®
• assay range: accuracy: 3-40,000 pg/mL; (Osteocrin); standard curve range: 0.6-9,000 pg/mL; (BDNF); standard curve range: 128-2,000,000 pg/mL; (Osteonectin (SPARC)); standard curve range: 16-250,000 pg/mL; (Oncostatin M); standard curve range: 22-350,000 pg/mL; (EPO); standard curve range: 256-4,000,000 pg/mL; (Myostatin); standard curve range: 26-400,000 pg/mL; (26Irisin); standard curve range: 3-40,000 pg/mL; (LIF); standard curve range: 3-40,000 pg/mL; (Osteocrin/Musclin (OSTN)); standard curve range: 48-750,000 pg/mL; (IL-15); standard curve range: 5-75,000 pg/mL; (FGF21); standard curve range: 9-140,000 pg/mL; (IL-6); inter-assay cv: <15%; intra-assay cv: <10%
• Technique(s): multiplexing: suitable
• Méthode de détection: fluorometric (Luminex xMAP)
• Conditions d'expédition: ambient

Description

Description générale

Skeletal muscle is actively involved in the synthesis and secretion of many proteins collectively termed “myokines.” Muscle contraction during physical activity is an important activator for the release of myokines, which act in an autocrine and/or paracrine manner to regulate skeletal muscle growth. Myokines also act as endocrine hormones which mediate inter-organ crosstalk. Myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue as well as for profound changes in immune and neuron systems. Various genetically engineered mouse strains and disease models are now serving as exceptional research tools for human myokine study.

MILLIPLEX^® Mouse Myokine Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture samples: Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO)*, Fibroblast Growth Factor 21 (FGF21), Follistatin-Like Protein 1 (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN / GDF8), Oncostatin M (OSM), Osteocrin/Musclin (OSTN), Osteonectin (SPARC).

*Only available for cells culture samples.

This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

• Analytes: Brain Derived Neurotrophic Factor (BDNF), Erythropoietin (EPO), Fibroblast Growth Factor 21 (FGF-21), Follistatin-like Protein 1 (FSTL1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
• NOTES: BDNF - Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF. OSM - While OSM is barely detectable in the serum/plasma of healthy subjects, a slight increase can be observed in certain disease states. Higher measurements in cell culture samples have also been reported. Erythropoeitin (EPO) is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma.
• Recommended Sample Type: Mouse serum, plasma, or cell/tissue culture supernatants or lysates
• Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
• Assay Run Time: For greater sensitivity an overnight incubation is recommended, (16-18 hours) at 2-8°C. Alternatively, incubate for 2 hours at room temperature (20-25°C).
• Research Category: Metabolism
• Research Subcategory: Obesity, Metabolic Disorders, Inflammation

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Autres remarques

Standard curve range: See protocol

Informations légales

6538 is a trademark of American Type Culture Collection

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H312 + H332,H317,H318,H373,H411
• Conseils de prudence: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Classification des risques: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Organes cibles: Respiratory Tract
• Classe de danger pour l'eau (WGK): WGK 3