MABC87

Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free)

clone Ab 1, from mouse

• Synonyme(s) : CD148, DEP-1, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, DEP1, HPTP eta, Protein-tyrosine phosphatase receptor type J, R-PTP-J
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: Ab 1, monoclonal
• Espèces réactives: human, mouse
• Technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001091973
• Numéro d'accès UniProt: Q12913
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PTPRJ(5795)

Description

Description générale

CD148, also known as Density-enhanced phosphatase 1 (DEP-1), is a membrane bound phosphatase that dephosphorylates a large number of protein targets and kinases thus serving as a regulator of many signaling cascades. CD148/DEP-1 plays a role in cell adhesion, migration, proliferation and differentiation. CD148/DEP-1 also plays a role in macrophage migration, adhesion and spreading. CD148/DEP-1 is also central to T-cell receptor signaling and acts as a negative regulator. CD148/DEP-1 acts as a positive regulator of endothelial cell survival and enhances the barrier function of epithelial junctions during reassembly. CD148/DEP-1 is also a key regulator of platelet activation and thrombosis as well as a tumor suppressor. CD148/DEP-1 is localized at the cell membrane particularly at cell projections, ruffle membranes, and cell junctions and it is expressed in lymphocytes and epithelial cells and expression increases as cell density increases.

Immunogène

His-tagged recombinant protein corresponding to Human CD148/DEP-1.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry (Paraffin) and Inhibition and Immunoprecipitation for the detection of CD148/DEP-1.

Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Raji, HEK293 +/-CD148 HA transfection, and A431D +/- CD148 transfection cell lysates.

Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Mouse kidney lysates (Takahashi, T., et al., (2006) Blood. 108:1234-1242).

Immunohistochemistry Analysis: A 1:50 dilution of a representative lot detected CD148/DEP-1 in Epithelial Cells of human prostate tissue.

Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in frozen mouse embryos. (Takahashi, T., et al., (2003) MOLECULAR AND CELLULAR BIOLOGY. 23(5):1817–1831).

Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in various mouse tissues such as kidney, cortex and cornea. (Takahashi, T., et al., (2006) Blood. 108:1234-1242).

Inhibition Assay: A representative lot of this antibody inhibited proliferation of HRMEC′s (Primary human renal microvascular endothelial cells) (Takahashi, T., et al., (2006) Blood. 108:1234-1242).

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated CD148/DEP-1 from Huvec and RMEC cell lysates (Takahashi, T., et al., (1999) J. Am. Soc. Nephrol. 10: 2135–2145).

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD148/DEP-1 in 10 µg of HeLa cell lysate.

Description de la cible

~260 kDa observed.
This protein is highly glycosylated and may run higher than the calculated molecular weight of 146 kDa (isoform 1) or 57 kDa (isoform 2). Uncharacterized bands may be observed in some lysates.

Forme physique

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable