ABF210

Anti-MDA5 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : MDA5 Antibody, Melanoma differentiation-associated protein 5, Interferon-induced with helicase C domain protein 1, IFIH1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès UniProt: Q9BYX4
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... IFIH1(64135)

Description

Description générale

Melanoma differentiation-associated protein 5 (MDA5), also known as Interferon-induced helicase C domain-containing protein 1 (IFIH1) is a member of the helicase family (RLR subfamily). MDA5 plays an important role in recognizing various viral components and triggers antiviral responses. When stimulated by dsRNA, MDA5 recruits adaptor protein VISA causing the activation of IRF-3 and NF-κB. MDA5 is widely expressed at low levels with barely detectable levels found in , testis and lung. Mutations in MDA5 have been associated with diabetes mellitus insulin-dependent type 19.

Spécificité

This antibody recognizes the C-terminus of MDA5.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human MDA5.

Application

Detect Melanoma differentiation-associated protein 5 using this rabbit polyclonal antibody, Anti-MDA5 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected MDA5 in human lymph node tissue lysate.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected MDA5 in human lymph node cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Qualité

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MDA5 in 15 µg of Daudi cell lysate.

Description de la cible

~117 kDa observed. Uncharacterized bands may appear in some lysates.

Liaison

Replaces: MABF198

Forme physique

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Daudi cell Lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable