AB4340
Anti-Zscan4 Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
Zinc finger and SCAN domain containing protein 4C
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Description générale
Zinc finger and SCAN domain containing protein 4C (UniProt: Q80VJ6) is encoded by the Zscan4c (also known as Gm397) gene (Gene ID: 245109) in mouse. Zscan4 is an embryonic stem (ES) cell-specific transcription factor required to regulate pluripotency, facilitate telomere elongation, and defy cellular senescence. It is expressed during zygotic genome activation in mouse two-cell embryos. It protects embryos from DNA damage by stabilizing the B-DNA form of microsatellite repeat DNA. It binds to the TGCACAC motif in CA/TG microsatellite repeats and regulates telomere elongation to maintain genomic stability and acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) in undifferentiated ES cells. Dysfunction of Zscan4 during development or in mESCs leads to implantation failure, telomere maintenance defects, and chromosomal rearrangements. (Ref.: Srinivasan, R., et al., (2020), Sci. Adv., 6, eaaz9115)
Immunogène
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of mouse Zscan4. The immunogen sequence is conserved between Zcsan4 family proteins (Zscan4b, Zscan4C, Zscan4d and Zscan4f).
Application
Anti-Zscan4, Cat. No. AB4340, is a rabbit polyclonal antibody that detects Zscan4 and is tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Qualité
Evaluated by Western Blotting in mouse embryonic stem cell (mESCs) lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Zscan4 in mouse embryonic stem cell (mESCs) lysate.
Description de la cible
Target molecular weight ~ 60 kDa observed; 60.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Recommended storage: +2°C to +8°C.
Remarque sur l'analyse
Control
Embryonic Stem Cell lysates
Embryonic Stem Cell lysates
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Lei Yang et al.
EMBO reports, 21(9), e50054-e50054 (2020-07-28)
Cloned animals generated by somatic cell nuclear transfer (SCNT) have been reported for many years; however, SCNT is extremely inefficient, and zygotic genome activation (ZGA) is required for SCNT-mediated somatic cell reprogramming. To identify candidate factors that facilitate ZGA in
Role of Tet proteins in enhancer activity and telomere elongation.
Lu, F; Liu, Y; Jiang, L; Yamaguchi, S; Zhang, Y
Genes & Development null
Oncostatin M Maintains Naïve Pluripotency of mESCs by Tetraploid Embryo Complementation (TEC) Assay.
Xiaoying Ye et al.
Frontiers in cell and developmental biology, 9, 675411-675411 (2021-06-15)
It has been well established that leukemia inhibitory factor (LIF) is essential for maintaining naïve pluripotency of embryonic stem cells (ESCs). Oncostatin M (OSM) is a member of the IL-6 family of cytokines which share gp130 as a receptor subunit
Yuqing Zhu et al.
Stem cell reports, 16(11), 2659-2673 (2021-10-09)
Mouse embryonic stem cells (ESCs) show cell-to-cell heterogeneity. A small number of two-cell-like cells (2CLCs) marked by endogenous retrovirus activation emerge spontaneously. The 2CLCs are unstable and they are prone to transiting back to the pluripotent state without extrinsic stimulus.
Diego Rodriguez-Terrones et al.
Nature genetics, 50(1), 106-119 (2017-12-20)
Unlike pluripotent cells, which generate only embryonic tissues, totipotent cells can generate a full organism, including extra-embryonic tissues. A rare population of cells resembling 2-cell-stage embryos arises in pluripotent embryonic stem (ES) cell cultures. These 2-cell-like cells display molecular features
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