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ZRB374

Sigma-Aldrich

Anti-GAPDH Antibody, clone 10B13, ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

EC: 1.2.1.12, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

rabbit (recombinant)

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

10B13, monoclonal
recombinant monoclonal

Description

10B13 Clone

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

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Cet article
H6666SRP3100F4537
KGF human Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

SRP3100

KGF human

biological source

human

biological source

human

biological source

human

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

≥95% (SDS-PAGE and HPLC)

assay

>95% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥97% (SDS-PAGE and N-terminal analysis)

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

100

form

powder

form

lyophilized powder

form

lyophilized

form

lyophilized powder

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 10B13 is a ZooMab rabbit recombinant monoclonal antibody that specifically detects human Glyceraldehyde-3-phosphate dehydrogenase and targets an epitope with in the C-terminal half.

Immunogène

GST/His-tagged recombinant fragment corresponding to 190 amino acids from the C-terminal half of human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Application

Anti-GAPDH, clone 10B13 ZooMAb, Cat. No. ZRB374, is a a recombinant Rabbit Monoclonal Antibody that specifically targets Glyceraldehyde-3-phosphate dehydrogenase and ha been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Wetern Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAPDH in HEK293 cell lysate.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.

Description de la cible

Glyceraldehyde-3-phosphate dehydrogenase (UniProt: P04406; also known as EC: 1.2.1.12, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH) is encoded by the GAPDH (also known as GAPD, CDABP0047, OK/SW-cl.1) gene (Gene ID: 2597) in human. GAPDH is considered as a ′house-keeping′ enzyme that catalyzes the synthesis of 1,3-biphosphoglycerate. It is a homotetrameric enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. Hence, it plays a role in both glycolysis and nuclear functions. It translocates to the nucleus following S-nitrosylation on cysteine 152 that leads to its interaction with SIAH1, which contains a nuclear localization signal. In the nucleus it participates in transcription, RNA transport, DNA replication and apoptosis. Sulfhydration at cysteine 152 is reported to increase its catalytic activity. Its nuclear functions are attributed to its nitrosylase activity, which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and DNA-PK. Oxidative stress is reported to promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process known as nucleocytoplasmic coagulation. These aggregates are observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at methionine 46 plays an essential role in the formation of these insoluble structures. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Besoin d'un modèle de COA ?

Ceci est un modèle de certificat d'analyse (COA pour Certificate of Analysis) et peut ne pas être représentatif d'un lot récemment fabriqué de ce produit spécifique.

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Tatiana I Slepak et al.
The Journal of biological chemistry, 299(9), 105105-105105 (2023-07-31)
CD97, an adhesion G-protein coupled receptor highly expressed in glioblastoma (GBM), consists of two noncovalently bound domains: the N-terminal fragment (NTF) and C-terminal fragment. The C-terminal fragment contains a GPCR domain that couples to Gα12/13, while the NTF interacts with

Protocoles

Follow this short antibody reconstitution protocol and see how easy it is to reconstitute ZooMAb® recombinant antibodies.

Follow this short antibody reconstitution protocol and see how easy it is to reconstitute ZooMAb® recombinant antibodies.

Follow this short antibody reconstitution protocol and see how easy it is to reconstitute ZooMAb® recombinant antibodies.

Contenu apparenté

Choisissez des options éco-responsables pour vos recherches avec les anticorps recombinants ZooMAb®, une alternative plus écologique. Ces anticorps sans BSA et sans transporteur sont expédiés dans un emballage à faible volume de déchets et offrent une stabilité maximale sans compromettre les performances.

Choisissez des options éco-responsables pour vos recherches avec les anticorps recombinants ZooMAb®, une alternative plus écologique. Ces anticorps sans BSA et sans transporteur sont expédiés dans un emballage à faible volume de déchets et offrent une stabilité maximale sans compromettre les performances.

Greener ZooMAb® antibodies offer sustainable options with low-waste packaging and BSA-free formulation.

Greener ZooMAb® antibodies offer sustainable options with low-waste packaging and BSA-free formulation.

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