684274

β1,2-Xylosidase, Xanthomonas sp.

• Synonyme(s) : β1,2-Xylosidase, Xanthomonas sp., β-D-Xylanxylohydrolase
• Numéro CAS: 9025-53-0
• Numéro de classification (Commission des enzymes): 3.2.1.37 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130640
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Forme: liquid
• Niveau de qualité: 100
• Activité spécifique: ≥10 units/mL; ≥20 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Activité étrangère: β-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases, none detected
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Native β1,2-Xylosidase from Xanthomonas species. Catalyses the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of a1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Note: 1 mU = 1 milliunit.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.

Forme physique

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Reconstitution

Following initial use, refrigerate (4°C).

Autres remarques

Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable