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X3876

Sigma-Aldrich

Xylanase from Trichoderma viride

greener alternative

lyophilized powder, 100-300 units/mg protein

Synonyme(s) :

1,4-β-D-Xylanxylanohydrolase, endo-1,4-β-Xylanase

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About This Item

Numéro CAS:
9025-57-4
Numéro de classification (Commission des enzymes):
3.2.1.8 (BRENDA, IUBMB)
Numéro CE :
232-800-2
Numéro MDL:
MFCD00132594
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

fungus (Trichoderma viride)

Niveau de qualité

200

Forme

lyophilized powder

Activité spécifique

100-300 units/mg protein

Poids mol.

22 kDa

Composition

Protein, ~50% biuret

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Activité étrangère

β-glucosidase and β-xylosidase ≤0.1%
cellulase <0.2%

Autre catégorie plus écologique

, Enabling

Température de stockage

2-8°C

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Description générale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research
Xylanase from Trichoderma viride was shown to have molecular weight of 22 kDa with a Pi of 9.3.
Xylanase is a hemicellulolytic enzyme. It is synthesized by microorganisms like bacteria, yeast and fungi.

Application

Xylanase from Trichoderma viride has been used as a component of an enzyme mixture for the hydrolysis of hemicellulose-rich solution (autohydrolysate). It has also been used as a cell-wall degrading enzyme to determine the efficiency of p-coumaryl esterase to release p-coumaroyl and feruloyl group.

Actions biochimiques/physiologiques

Xylanase (endo-1,4-β-Xylanase) is involved in the hydrolysis of xylan. It has a wide range of applications in industrial processes such as, bioleeching of craft pulp in paper industry and production of hydrolysates from agro-industrial wastes. Xylanase is also used in nutritional enhancement of lignocellulosic feed and in clarification of juice and wines.

Définition de l'unité

One unit will liberate 1 μmole of 4-nitrophenol from 4-nitrophenol-xylan per min at pH 4.5 at 30 °C.

Forme physique

Contains sorbitol and sodium acetate buffer salts

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Bioresource technology, 102(2), 1234-1239 (2010-08-28)
Oil palm (Elaeis guineensis Jacq.) is one of the most important commercial crops for the production of palm oil, which generates 10.88 tons of oil palm fronds per hectare of plantation as a by-product. In this study, oil palm frond
Low-molecular-weight xylanase from Trichoderma viride
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Applied and Environmental Microbiology, 57, 1860-1862 (1994)
Agnieszka Wikiera et al.
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The biological activity of apple pectin extracted conventionally or enzymatically using endo-xylanase and endo-cellulase, was tested in vitro. The analyses were performerd in tetraplicates and the statistical significance of the differences were assessed using ANOVA, Tukey post hoc and LSD
Meenakshi Goyal et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 39(3), 535-541 (2008-07-01)
In the present study, cultural and nutritional conditions for enhanced production of xylanase by a local soil isolate of Trichoderma viride, using various lignocellulosic substrates in submerged culture fermentation have been optimized. Of the lignocellulosics used, maize straw was the
M L T M Polizeli et al.
Applied microbiology and biotechnology, 67(5), 577-591 (2005-06-10)
Xylan is the principal type of hemicellulose. It is a linear polymer of beta-D-xylopyranosyl units linked by (1-4) glycosidic bonds. In nature, the polysaccharide backbone may be added to 4-O-methyl-alpha-D-glucuronopyranosyl units, acetyl groups, alpha-L-arabinofuranosyl, etc., in variable proportions. An enzymatic
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