07-632

Anti-NR2A Antibody

Upstate^®, from rabbit

• Synonyme(s) : N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor channe, subunit epsilon-1, N-methyl-D-aspartate receptor subunit 2A, NMDA receptor subtype 2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: rat, mouse
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NM_008170
• Numéro d'accès UniProt: Q12879
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GRIN2A(2903)

Description

Description générale

NR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Spécificité

Recognizes NR2A, MW ~170 kDa.

Immunogène

6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A).

Application

Detect NR2A using this Anti-NR2A Antibody validated for use in IP & WB.

Immunoprecipitation:
4 µg of a previous lot immuno-precipitated NR2A from rat brain hippocampus membranes and rat brain microsomal preparations.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Qualité

Evaluated by western blot in rat brain microsomal preparations (Catalog # 12-144).

Western Blot Analysis:
0.5-2 µg/mL of this antibody detected NR2A in rat brain microsomal preparations (Catalog # 12-144).

Description de la cible

~170 kDa

Forme physique

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.

Stockage et stabilité

Stable for 1 year at -20ºC from date of receipt.

Remarque sur l'analyse

Control
Brain tissue.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable