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Merck
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Key Documents

07-482

Sigma-Aldrich

Anti-Calmodulin Binding Protein Epitope Tag Antibody

Upstate®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

E. coli

Fabricant/nom de marque

Upstate®

Technique(s)

western blot: suitable

Isotype

IgG

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase.

Spécificité

Recognizes recombinant proteins containing the calmodulin binding protein epitope tag.

Immunogène

KLH-conjugated, synthetic peptide (MKRRWKKNFIAVSAANRFKKISSSGAL) corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors. The immunizing sequence is identical in rabbit, mouse, and rat.

Application

Anti-Calmodulin Binding Protein Epitope Tag Antibody detects level of Calmodulin Binding Protein Epitope Tag & has been published & validated for use in WB.

Qualité

Routinely evaluated by Western Blot in lysates from transformed E. coli.

Western Blot Analysis:
A 1:2000-1:10000 dilution of this lot detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.

Description de la cible

The molecular weight is related to the tagged protein.

Forme physique

Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4.

Remarque sur l'analyse

Control
Lysates from transformed E. coli.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Weijun Chen et al.
RNA (New York, N.Y.), 20(3), 308-320 (2014-01-21)
Excision of introns from pre-mRNAs is mediated by the spliceosome, a multi-megadalton complex consisting of U1, U2, U4/U6, and U5 snRNPs plus scores of associated proteins. Spliceosome assembly and disassembly are highly dynamic processes involving multiple stable intermediates. In this
Role of km23-1 in RhoA/actin-based cell migration.
Jin, Q; Pulipati, NR; Zhou, W; Staub, CM; Liotta, LA; Mulder, KM
Biochemical and biophysical research communications null
Stephen M T Hoke et al.
Current genetics, 56(5), 447-465 (2010-07-17)
Tra1 is a component of the Saccharomyces cerevisiae SAGA and NuA4 complexes and a member of the PIKK family, which contain a C-terminal phosphatidylinositol 3-kinase-like (PI3K) domain followed by a 35-residue FATC domain. Single residue changes of L3733A and F3744A
Ingo Bauer et al.
mBio, 7(6) (2016-11-03)
Histone deacetylases (HDACs) remove acetyl moieties from lysine residues at histone tails and nuclear regulatory proteins and thus significantly impact chromatin remodeling and transcriptional regulation in eukaryotes. In recent years, HDACs of filamentous fungi were found to be decisive regulators
Angelo Pidroni et al.
Frontiers in microbiology, 9, 2212-2212 (2018-10-05)
An outstanding feature of filamentous fungi is their ability to produce a wide variety of small bioactive molecules that contribute to their survival, fitness, and pathogenicity. The vast collection of these so-called secondary metabolites (SMs) includes molecules that play a

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