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Key Documents

05-531

Sigma-Aldrich

Anti-His Tag Antibody

clone 4D11, mouse monoclonal, Upstate

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43

product name

Anti-Histidine Tagged Antibody, clone 4D11, clone 4D11, Upstate®, from mouse

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

4D11, monoclonal

Réactivité de l'espèce (prédite par homologie)

all

Fabricant/nom de marque

Upstate®

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Isotype

IgG2aκ

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HRG(3273)

Spécificité

Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag

Immunogène

N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.

Application

This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.

Qualité

routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins

Description de la cible

The apparent molecular weight is related to the tagged protein.

Forme physique

Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.

Remarque sur l'analyse

Control
Cells expressing the His-tag

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Ruiping Zheng et al.
Journal of cell science, 123(Pt 21), 3734-3744 (2010-10-14)
In higher eukaryotic cells, long non-protein-coding RNAs (lncRNAs) have been implicated in a wide array of cellular functions. Cell- or tissue-specific expression of lncRNA genes encoded in the mammalian genome is thought to contribute to the complex gene networks needed
Lisa A Mitchell et al.
Molecular human reproduction, 14(4), 235-243 (2008-02-05)
The process of capacitation is a pre-requisite for mammalian spermatozoa allowing them to gain the ability to fertilize an oocyte. A fundamental part of this mechanism is a dramatic increase in the level of tyrosine phosphorylation. Implicated in this process
K J Robson et al.
The EMBO journal, 14(16), 3883-3894 (1995-08-15)
Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much
Francesca Mancini et al.
The EMBO journal, 28(13), 1926-1939 (2009-06-13)
MDM4 is a key regulator of p53, whose biological activities depend on both transcriptional activity and transcription-independent mitochondrial functions. MDM4 binds to p53 and blocks its transcriptional activity; however, the main cytoplasmic localization of MDM4 might also imply a regulation
Wei-Hang Huang et al.
Heliyon, 9(11), e21486-e21486 (2023-11-29)
Originally extracted from Momordica charantia seeds, the antiviral and anti-tumor activities of Momordica anti-HIV protein MAP30 have become well known. Although MAP30 has been reported to possess antiviral activity against several human viruses, the current understanding of the MAP30-mediated antiviral

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